Interaction of G-Quadruplexes in the Full-Length 3' Human Telomeric Overhang.
The 3' human telomeric overhang provides ample opportunities for the formation and interaction of G-quadruplexes, which have shown impacts on many biological functions including telomerase activities in the telomere region. However, in the few investigations on DNA constructs that approach to the fu...
| Publicado en: | Journal of the American Chemical Society Vol. 136; no. 52; pp. 18062 - 18070 |
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| Autores principales: | , , , , , , |
| Formato: | Artículo |
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American Chemical Society
12/31/2014
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| Materias: | |
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=hlh&AN=100583477&site=ehost-live header: @attributes: shortDbName: hlh uiTerm: 100583477 longDbName: Humanities International Complete uiTag: AN controlInfo: bkinfo: jinfo: jid: 00027863 ACS jtl: Journal of the American Chemical Society issn: 00027863 maglogo: N pubinfo: dt: 12/31/2014 vid: 136 iid: 52 pid: 997 pub: American Chemical Society artinfo: ui: 100583477 10.1021/ja510079u ppf: 18062 ppct: 8 formats: tig: atl: Interaction of G-Quadruplexes in the Full-Length 3' Human Telomeric Overhang. aug: au: Abraham Punnoose, Jibin Yurnri Cui Deepak Koirala Yangyuoru, Philip M. Ghimire, Chiran Shrestha, Prakash Hanbin Mao affil: Department of Chemistry and Biochemistry, Kent State University, Kent, Ohio 44242, United States su: Quadruplex nucleic acids Telomerase Ligation reactions DNA structure Guanine Telomeres Tandem repeats Hydrogen bonding sug: subj: Quadruplex nucleic acids Telomerase Ligation reactions DNA structure Guanine Telomeres Tandem repeats Hydrogen bonding ab: The 3' human telomeric overhang provides ample opportunities for the formation and interaction of G-quadruplexes, which have shown impacts on many biological functions including telomerase activities in the telomere region. However, in the few investigations on DNA constructs that approach to the full length of the human telomeric overhang, the presence of higher-order quadruplex--quadruplex interactions is still a subject of debate. Herein, we employed dynamic splint ligation (DSL) to prepare a DNA construct, 5'-(TTAGGG)24 or 24G, which has the length comparable to the full stretch of 3' human telomeric overhang. Using mechanical unfolding assays in laser tweezers, we observed a minor population (~5%) of higher-order interactions between G-quadruplexes, while the majority of the quadruplexes follow the bead-on-a-string model. Analyses on the noninteracting G-quadruplexes in the 24G construct showed features similar to those of the stand-alone G-quadruplexes in the 5'-(TTAGGG)4 (4G) construct. As each 24G construct contains as many as six G-quadruplexes, this method offers increased throughput for the time-consuming mechanical unfolding experiments of non-B DNA structures. pubtype: Academic Journal doctype: Article src: R language: English refInfo: copyright: @attributes: flag: Y dt: @attributes: year: 2014 holdings: @attributes: islocal: N |
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