Pathogenic and enzyme activities of the entomopathogenic fungus Tolypocladium cylindrosporum (Ascomycota: Hypocreales) from Tierra del Fuego, Argentina.
Tolypocladium cylindrosporum is an entomopathogenic fungi that has been studied as a biological control agent against insects of several orders. The fungus has been isolated from the soil as well as from insects of the orders Coleoptera, Lepidoptera, Diptera and Hymenoptera. In this study, we analyz...
| Publicado en: | Revista de Biología Tropical Vol. 60; no. 2; pp. 833 - 842 |
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| Autores principales: | , , , , , |
| Formato: | Artículo |
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Universidad de Costa Rica
jun2012
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| Materias: | |
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=lth&AN=102118466&site=ehost-live header: @attributes: shortDbName: lth uiTerm: 102118466 longDbName: MedicLatina uiTag: AN controlInfo: bkinfo: jinfo: jid: 00347744 2BO jtl: Revista de Biología Tropical issn: 00347744 maglogo: N pubinfo: dt: jun2012 vid: 60 iid: 2 pid: 4413 pub: Universidad de Costa Rica artinfo: ui: 102118466 10.15517/rbt.v60i2.4006 ppf: 833 ppct: 9 formats: tig: atl: Pathogenic and enzyme activities of the entomopathogenic fungus Tolypocladium cylindrosporum (Ascomycota: Hypocreales) from Tierra del Fuego, Argentina. aug: au: Scorsetti, Ana C. Elíades, Lorena A. Stenglein, Sebastián A. Cabello, Marta N. Pelizza, Sebastián A. Saparrat, Mario C. N. affil: Instituto de Botánica Carlos Spegazzini (FCNyM-UNLP) 53 # 477, (1900), La Plata, Argentina Laboratorio de Biología Funcional y Biotecnología (BIOLAB)-CEBB-CONICET, Cátedra de Microbiología, Facultad de Agronomía de Azul, UNCPBA, República de Italia # 780, Azul (7300), Argentina Comisión de Investigaciones Científicas de la provincia de Buenos Aires Centro de Estudios Parasitológicos y de Vectores (CEPAVE), CCT-La Plata-CONICET-UNLP, Calle 2 # 584, La Plata (1900), Argentina Instituto de Fisiología Vegetal (INFIVE), Universidad Nacional de La Plata (UNLP)- CCT-La Plata- Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Diag. 113 y 61, CC 327, 1900-La Plata, Argentina Cátedra de Microbiología Agrícola, Facultad de Ciencias Agrarias y Forestales, UNLP, 60 y 119, 1900-La Plata, Argentina su: Entomopathogenic fungi Biological pest control Insects Beetles Lepidoptera Diptera Hymenoptera sug: subj: Entomopathogenic fungi Biological pest control Insects Beetles Lepidoptera Diptera Hymenoptera keyword: Biological control chitinolytic activity entomopathogenesis hydrolases soil microorganisms Tolypocladium cylindrosporum actividad quitinolítica control biológico entomopatogénesis hidrolasas microorganismos de suelo ab: Tolypocladium cylindrosporum is an entomopathogenic fungi that has been studied as a biological control agent against insects of several orders. The fungus has been isolated from the soil as well as from insects of the orders Coleoptera, Lepidoptera, Diptera and Hymenoptera. In this study, we analyzed the ability of a strain of T. cylindrosporum, isolated from soil samples taken in Tierra del Fuego, Argentina, to produce hydrolytic enzymes, and to study the relationship of those activities to the fungus pathogenicity against pest aphids. We have made the traditional and molecular characterization of this strain of T. cylindrosporum. The expression of hydrolase activity in the fungal strain was estimated at three incubation temperatures (4ºC, 12ºC and 24ºC), on different agar media supplemented with the following specific substrates: chitin azure, Tween ® 20, casein, and urea for chitinase, lipase, protease, and urease activity, respectively. The hydrolytic-enzyme activity was estimated qualitatively according to the presence of a halo of clarification through hydrolase action, besides was expressed semi-quantitatively as the ratio between the hydrolytic-halo and colony diameters. The pathogenicity of the fungus was tested on adults of the aphid Rhopalosiphum padi at three temperatures of incubation (4ºC, 12ºC and 24ºC). The suspension was adjusted to a concentration of 1x10 7 conidia/ml. In pathogenicity assays at seven days post-inoculation, the fungus caused the mortality of adults of Ropalosiphum padi at different temperatures also showed a broad ability to grow on several agar-culture media, supplemented with different carbon sources at the three incubation temperatures tested. Although, the growth was greater with higher incubation temperatures (with maximum levels at 24°C), the fungus reached similar colony diameters after 15 days of incubation on the medium supplemented with Tween® 20 at the lower two incubation temperatures of 4°C or 12°C. In accordance with the results on colony diameters, the fungus revealed an ability to degrade casein, chitin derivatives, Tween® 20, and urea as evidenced by the appearance of a halo around the fungal colony. Because of its origin and temperature tolerance, this Argentine strain has great potential for use as a biocontrol agent for insect pest control in cold and temperate environments. El hongo entomopatógeno Tolypocladium cylindrosporum ha sido estudiado como un agente de control biológico contra insectos de varios órdenes. Esta especie fue aislada del suelo, así como de insectos de los órdenes Coleoptera, Lepidoptera, Diptera e Hymenoptera. En el presente trabajo hemos analizado la capacidad de una cepa de T. cylindrosporum (LPSC Nº1065) aislada del suelo en Tierra del Fuego, Argentina, para producir enzimas hidrolíticas y determinar la relación de esta actividad con la patogenicidad del hongo para combatir la plaga de los áfidos en diferentes temperaturas (4º, 12º y 24ºC). En los ensayos de patogenicidad, siete días posteriores a la inoculación, se registró mortalidad en los adultos del áfido Ropalosiphum padi a diferentes temperaturas y también se demostró una amplia capacidad de crecer en varios medios de cultivos complementados con diferentes fuentes de carbono bajo las tres temperaturas de incubación ensayadas. Debido a su origen y a la tolerancia que tiene a bajas temperaturas esta cepa, presenta un gran potencial para su uso como agente de control biológico para las plagas de insectos de ambientes fríos y templados. pubtype: Academic Journal doctype: Article src: R language: English refInfo: copyright: @attributes: flag: Y dt: @attributes: year: 2012 holdings: @attributes: islocal: N |
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