Simultaneous dermal matrix and autologous split-thickness skin graft transplantation in a porcine wound model: A three-dimensional histological analysis of revascularization.
Despite the popularity of a simultaneous application of dermal matrices and split-thickness skin grafts, scarce evidence exists about the process of revascularization involved. In this study, we aimed at analyzing the progression of revascularization by high-resolution episcopic microscopy ( HREM) i...
| Publicado en: | Wound Repair & Regeneration Vol. 22; no. 6; pp. 749 - 755 |
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| Autores principales: | , , , , , , , , |
| Formato: | pictorial research Journal Article |
| Publicado: |
Wiley-Blackwell
Nov/Dec2014
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=103745387&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 103745387 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 10671927 DPV jtl: Wound Repair & Regeneration issn: 10671927 maglogo: Y pubinfo: dt: Nov/Dec2014 vid: 22 iid: 6 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 103745387 100631436 10.1111/wrr.12233 NLM25358670 103745387 ppf: 749 ppct: 6 formats: fmt: @attributes: type: P tig: atl: Simultaneous dermal matrix and autologous split-thickness skin graft transplantation in a porcine wound model: A three-dimensional histological analysis of revascularization. aug: au: Wiedner, Maria Tinhofer, Ines E. Kamolz, Lars-Peter Seyedian Moghaddam, Atieh Justich, Ivo Liegl-Atzwanger, Bernadette Bubalo, Vladimir Weninger, Wolfgang J. Lumenta, David B. affil: Research Unit for Tissue Regeneration, Repair, and Reconstruction, Division of Plastic, Aesthetic, and Reconstructive Surgery, Department of Surgery, Medical University of Graz sug: subj: Autografts Skin Transplantation Wounds and Injuries Surgery Neovascularization, Physiologic Animal Studies Swine Models, Biological Biopsy Descriptive Statistics Paired T-Tests Wilcoxon Rank Sum Test Post Hoc Analysis Data Analysis Software ab: Despite the popularity of a simultaneous application of dermal matrices and split-thickness skin grafts, scarce evidence exists about the process of revascularization involved. In this study, we aimed at analyzing the progression of revascularization by high-resolution episcopic microscopy ( HREM) in a porcine excisional wound model. Following the surgical procedure creating 5 × 5 cm2 full-thickness defects on the back, one area was covered with an autologous split-thickness skin graft alone (control group), the other with a collagen-elastin dermal matrix plus split-thickness skin graft (dermal matrix group). Two skin biopsies per each group and location were performed on day 5, 10, 15, and 28 postoperatively and separately processed for H& E as well as HREM. The dermal layer was thicker in the dermal matrix group vs. control on day 5 and 28. No differences were found for revascularization by conventional histology. In HREM, the dermal matrix did not appear to decelerate the revascularization process. The presence of the dermal matrix could be distinguished until day 15. By day 28, the structure of the dermal matrix could no longer be delineated and was replaced by autologous tissue. As assessed by conventional histology and confirmed by HREM, the revascularization process was comparable in both groups, notably with regard to the vertical ingrowth of sprouting vessels. The presented technique of HREM is a valuable addition for analyzing small vessel sprouting in dermal matrices in the future. pubtype: Academic Journal doctype: pictorial research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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