Differential Effects of Ethanol on Bid, tBid, and Bax: tBid Interactions in Postnatal Day 4 and Postnatal Day 7 Rat Cerebellum.
Background Exposure to ethanol (EtOH) during central nervous system (CNS) development can lead to a wide array of neuroanatomical, behavioral, and cognitive abnormalities, broadly subsumed under the fetal alcohol spectrum disorder classification. One mode of EtOH-induced interference in the normal d...
| Publicado en: | Alcoholism: Clinical & Experimental Research Vol. 39; no. 1; pp. 55 - 64 |
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| Autores principales: | , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
Jan2015
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=103745824&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 103745824 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 01456008 IQE jtl: Alcoholism: Clinical & Experimental Research issn: 01456008 maglogo: Y pubinfo: dt: Jan2015 vid: 39 iid: 1 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 103745824 100641298 100641298 10.1111/acer.12603 103745824 ppf: 55 ppct: 9 formats: tig: atl: Differential Effects of Ethanol on Bid, tBid, and Bax: tBid Interactions in Postnatal Day 4 and Postnatal Day 7 Rat Cerebellum. aug: au: Heaton, Marieta B. Paiva, Michael Kubovec, Stacey affil: Department of Neuroscience, University of Florida College of Medicine; McKnight Brain Institute; Center for Addiction Research and Education sug: subj: Cerebellum Drug Effects Ethanol Administration and Dosage Fetal Alcohol Syndrome Pathology Apoptosis Drug Effects Proteins Drug Effects Animal Studies Rats Oncogenes Enzyme-Linked Immunosorbent Assay In Vitro Studies Florida Ethanol Blood Cell Culture Techniques Biological Assay T-Tests Analysis of Variance Post Hoc Analysis Data Analysis Software Descriptive Statistics Neurons Funding Source ab: Background Exposure to ethanol (EtOH) during central nervous system (CNS) development can lead to a wide array of neuroanatomical, behavioral, and cognitive abnormalities, broadly subsumed under the fetal alcohol spectrum disorder classification. One mode of EtOH-induced interference in the normal developmental program appears to be through induction of apoptotic processes mediated by the Bcl-2 family of survival-regulatory proteins. The present series of studies investigated the role of the Bcl-2-related, pro-apoptotic Bid protein, and its truncated, apoptotically active fragment, tBid, in developmental EtOH neurotoxicity. Methods Protein analyses were made via enzyme-linked immunosorbent assays (ELISA) in neonatal rat cerebellum, of basal Bid, and of Bid and tBid, following EtOH exposure via vapor inhalation, at an age of peak EtOH sensitivity in this region (postnatal day 4 [P4]) and a later age of relative resistance (P7). ELISA analyses were also made of Bax: tBid heterodimers, a process which activates Bax, essential for its apoptotic functioning. Finally, in vitro assessments of the importance of tBid to EtOH neurotoxicity were made in cultured cerebellar granule cells, using a specific tBid inhibitor. Results Basal levels of Bid were higher at P4 compared to P7, possibly contributing to the differential sensitivity. EtOH exposure elicited further increases in cytosolic Bid and mitochondrial tBid when administration was at P4, but not at P7. Bax: tBid heterodimers were markedly increased by EtOH exposure on P4, an increase which persisted even 2 hours after termination of treatment. Similar effects were not seen at P7. The in vitro analyses revealed that tBid inhibition provided complete protection against EtOH-induced cell death and depressed EtOH-mediated cytochrome-c release. Conclusions These results suggest that Bid/ tBid may be important elements in EtOH-mediated neurotoxicity during CNS development. The molecular processes and interactions revealed may represent critical points which can be targeted in studies concerned with designing possible therapeutic strategies for minimizing these devastative effects. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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