Selective antibacterial activity of patchouli alcohol against Helicobacter pylori based on inhibition of urease.
The aim of this study is to evaluate the antibacterial activity and urease inhibitory effects of patchouli alcohol (PA), the bioactive ingredient isolated from Pogostemonis Herba, which has been widely used for the treatment of gastrointestinal disorders. The activities of PA against selected bacter...
| Publicado en: | Phytotherapy Research Vol. 29; no. 1; pp. 67 - 73 |
|---|---|
| Autores principales: | , , , , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Wiley-Blackwell
Jan2015
|
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=103747697&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 103747697 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 0951418X O9B jtl: Phytotherapy Research issn: 0951418X maglogo: Y pubinfo: dt: Jan2015 vid: 29 iid: 1 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 103747697 NLM25243578 2012889887 10.1002/ptr.5227 NLM25243578 103747697 ppf: 67 ppct: 6 formats: tig: atl: Selective antibacterial activity of patchouli alcohol against Helicobacter pylori based on inhibition of urease. aug: au: Yu, Xiao-Dan Xie, Jian-Hui Wang, Yong-Hong Li, Yu-Cui Mo, Zhi-Zhun Zheng, Yi-Feng Su, Ji-Yan Liang, Ye-Er Liang, Jin-Zhi Su, Zi-Ren Huang, Ping affil: College of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, 510006, PR China. sug: subj: Antibiotics Pharmacodynamics Helicobacter Pylori Drug Effects Plants Hydrocarbons Pharmacodynamics Urease Antagonists and Inhibitors Amoxicillin Pharmacodynamics Helicobacter Pylori Microbial Culture and Sensitivity Tests Plant Extracts ab: The aim of this study is to evaluate the antibacterial activity and urease inhibitory effects of patchouli alcohol (PA), the bioactive ingredient isolated from Pogostemonis Herba, which has been widely used for the treatment of gastrointestinal disorders. The activities of PA against selected bacteria and fungi were determined by agar dilution method. It was demonstrated that PA exhibited selective antibacterial activity against Helicobacter pylori, without influencing the major normal gastrointestinal bacteria. Noticeably, the antibacterial activity of PA was superior to that of amoxicillin, with minimal inhibition concentration value of 78 µg/mL. On the other hand, PA inhibited ureases from H.pylori and jack bean in concentration-dependent fashion with IC50 values of 2.67 ± 0.79 mM and 2.99 ± 0.41 mM, respectively. Lineweaver-Burk plots indicated that the type of inhibition was non-competitive against H.pylori urease whereas uncompetitive against jack bean urease. Reactivation of PA-inactivated urease assay showed DL-dithiothreitol, the thiol reagent, synergistically inactivated urease with PA instead of enzymatic activity recovery. In conclusion, the selective H.pylori antibacterial activity along with urease inhibitory potential of PA could make it a possible drug candidate for the treatment of H.pylori infection. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|