Phellinus Linteus Extract Induces Autophagy and Synergizes With 5-Fluorouracil to Inhibit Breast Cancer Cell Growth.
Phellinus linteus(PL) is a medicinal mushroom due to its several biological properties, including anticancer activity. However, the mechanisms of its anticancer effect remain to be elucidated. We evaluated the inhibitory effects of the ethanolic extract from the PL combined with 5-FU on MDA-MB-231 b...
| Publicado en: | Nutrition & Cancer Vol. 67; no. 2; pp. 275 - 285 |
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| Autores principales: | , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Taylor & Francis Ltd
Feb/Mar2015
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=103765243&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 103765243 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 01635581 7MS jtl: Nutrition & Cancer issn: 01635581 maglogo: N pubinfo: dt: Feb/Mar2015 vid: 67 iid: 2 pid: 377 pub: Taylor & Francis Ltd place: Philadelphia, Pennsylvania artinfo: ui: 103765243 101347556 10.1080/01635581.2015.989374 NLM25622112 103765243 ppf: 275 ppct: 10 formats: fmt: @attributes: type: P tig: atl: Phellinus Linteus Extract Induces Autophagy and Synergizes With 5-Fluorouracil to Inhibit Breast Cancer Cell Growth. aug: au: Lee, Wen-Ying Hsu, Keng-Fu Chiang, Tai-An Chen, Chee-Jen affil: Department of Pathology, Chi Mei Medical Center, Tainan, Taiwan;Department of Pathology, College of Medicine, Taipei Medical University, Taipei, Taiwan; andDepartment of Medical Laboratory Science and Biotechnology, Chung Hwa University of Medical Technology, Tainan, Taiwan sug: subj: Plants, Medicinal Therapeutic Use Plant Extracts Autophagy Fluorouracil Therapeutic Use Breast Neoplasms Prevention and Control Cell Physiology Nutrition Serial Publications Human Female Academic Medical Centers Taiwan Treatment Failure Evaluation Neoplasm Metastasis Complications Outcomes (Health Care) Chemotherapy, Adjuvant Drug Toxicity Diagnosis Cell Culture Techniques Apoptosis Cell Cycle Staining and Labeling Microscopy, Electron Descriptive Statistics Control Group Female ab: Phellinus linteus(PL) is a medicinal mushroom due to its several biological properties, including anticancer activity. However, the mechanisms of its anticancer effect remain to be elucidated. We evaluated the inhibitory effects of the ethanolic extract from the PL combined with 5-FU on MDA-MB-231 breast cancer cell line and to determine the mechanism of cell death. Individually, PL extract and 5-FU significantly inhibited the proliferation of MDA-MB-231 cells in a dose-dependent manner. PL extract (30 mg/mL) in combination with 5-FU (10 μg/mL) synergistically inhibited MDA-MB-231 cells by 1.8-fold. PL did not induce apoptosis, as demonstrated by the DNA fragmentation assay, the sub-G1 population, and staining with annexin V-FITC and propidium iodide. The exposure of MDA-MB-231 cells to PL extracts resulted in several confirmed characteristics of autophagy, including the appearance of autophagic vacuoles revealed by monodansylcadaverine staining, the formation of acidic vesicular organelles, autophagosome membrane association of microtubule-associated protein light chain 3 (LC3) characterized by cleavage of LC3 and its punctuate redistribution, and ultrastructural observation of autophagic vacuoles by transmission electron microscopy. We concluded that PL extracts synergized with low doses of 5-FU to inhibit triple-negative breast cancer cell growth and demonstrated that PL extract can induce autophagy-related cell death. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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