Comparison of optical coherence tomography and histopathology in quantitative assessment of goat talus articular cartilage.
Background and purpose - Optical coherence tomography (OCT) is a light-based imaging technique suitable for depiction of thin tissue layers such as articular cartilage. Quantification of results and direct comparison with a reference standard is needed to confirm the role of OCT in cartilage evaluat...
| Publicado en: | Acta Orthopaedica Vol. 86; no. 2; pp. 257 - 264 |
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| Autores principales: | , , , , , , , , |
| Formato: | diagnostic images research tables/charts Journal Article |
| Publicado: |
Medical Journals Sweden AB
Apr2015
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=103780575&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 103780575 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 17453674 1BVL jtl: Acta Orthopaedica issn: 17453674 maglogo: N pubinfo: dt: Apr2015 vid: 86 iid: 2 pid: 59195 pub: Medical Journals Sweden AB artinfo: ui: 103780575 101735221 10.3109/17453674.2014.979312 NLM25350610 103780575 ppf: 257 ppct: 7 formats: fmt: @attributes: type: P tig: atl: Comparison of optical coherence tomography and histopathology in quantitative assessment of goat talus articular cartilage. aug: au: Cernohorsky, Paul Kok, Aimee C Bruin, Daniel Martijn de Brandt, Martin J Faber, Dirk J Tuijthof, Gabrielle J Kerkhoffs, Gino M Strackee, Simon D van Leeuwen, Ton G affil: Plastic, Reconstructive and Hand Surgery sug: subj: Tomography, Optical Coherence Cartilage, Articular Surgery Talus Surgery Talus Pathology Cartilage, Articular Pathology Animal Studies Mammals Data Analysis Software Descriptive Statistics Correlation Coefficient Treatment Outcomes Bone Diseases Surgery Intrarater Reliability Paired T-Tests Confidence Intervals ab: Background and purpose - Optical coherence tomography (OCT) is a light-based imaging technique suitable for depiction of thin tissue layers such as articular cartilage. Quantification of results and direct comparison with a reference standard is needed to confirm the role of OCT in cartilage evaluation. Materials and methods - Goat talus articular cartilage repair was assessed quantitatively with OCT and compared with histopathology using semi-automated analysis software. Osteochondral defects were created centrally in goat tali with subsequent healing over 24 weeks. After sacrifice, the tali were analyzed using OCT and processed into histopathology slides. Cartilage thickness, repair tissue area, and surface roughness were measured. Also, light attenuation coefficient measurements were performed to assess differences in the properties of healthy tissue and repair tissue. Results - Intra-class correlation coefficients for resemblance between the 2 techniques were 0.95 (p < 0.001) for thickness, 0.73 (p = 0.002) for repair tissue area, and 0.63 (p = 0.015) for surface roughness. Light attenuation differed significantly between healthy cartilage (8.2 (SD 3.9) mm-1) and repair tissue (2.8 (SD 1.5) mm-1) (p < 0.001). Interpretation - Compared to histopathology as the standard reference method, OCT is a reproducible technique in quantitative analysis of goat talus articular cartilage, especially when assessing cartilage thickness and to a lesser extent when measuring repair tissue area and surface roughness. Moreover, differences in local light attenuation suggest measurable variation in tissue structure, enhancing the clinical applicability of quantitative measurements from cartilage OCT images. pubtype: Academic Journal doctype: diagnostic images research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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