Expansion of urease- and uricase-containing, indole- and p-cresol-forming and contraction of short-chain fatty acid-producing intestinal microbiota in ESRD.
Background: Intestinal microbiome constitutes a symbiotic ecosystem that is essential for health, and changes in its composition/function cause various illnesses. Biochemical milieu shapes the structure and function of the microbiome. Recently, we found marked differences in the abundance of numerou...
| Publicado en: | American Journal of Nephrology Vol. 39; no. 2; pp. 230 - 238 |
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| Autores principales: | , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Karger AG
Mar2014
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=103817216&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 103817216 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 02508095 8BL jtl: American Journal of Nephrology issn: 02508095 maglogo: N pubinfo: dt: Mar2014 vid: 39 iid: 2 pid: 2485 pub: Karger AG artinfo: ui: 103817216 103817216 NLM24643131 2012543228 10.1159/000360010 NLM24643131 PMC4049264 103817216 ppf: 230 ppct: 8 formats: tig: atl: Expansion of urease- and uricase-containing, indole- and p-cresol-forming and contraction of short-chain fatty acid-producing intestinal microbiota in ESRD. aug: au: Wong, Jakk Piceno, Yvette M Desantis, Todd Z Pahl, Madeleine Andersen, Gary L Vaziri, Nosratola D affil: Center for Environmental Biotechnology, Lawrence Berkeley National Laboratory, Berkeley, Calif., USA. sug: subj: Phenols Fatty Acids, Volatile Indoles Kidney Failure, Chronic Metabolism Oxidoreductases Urease Microbiota Adult Aged Ammonia Diet Female Human Inflammation Intestines Microbiology Kidney Failure, Chronic Microbiology Male Microbiologic Phenomena Middle Age Sulfuric Acids Urea Adult: 19-44 years Aged: 65+ years Middle Aged: 45-64 years Female Male ab: Background: Intestinal microbiome constitutes a symbiotic ecosystem that is essential for health, and changes in its composition/function cause various illnesses. Biochemical milieu shapes the structure and function of the microbiome. Recently, we found marked differences in the abundance of numerous bacterial taxa between ESRD and healthy individuals. Influx of urea and uric acid and dietary restriction of fruits and vegetables to prevent hyperkalemia alter ESRD patients' intestinal milieu. We hypothesized that relative abundances of bacteria possessing urease, uricase, and p-cresol- and indole-producing enzymes is increased, while abundance of bacteria containing enzymes converting dietary fiber to short-chain fatty acids (SCFA) is reduced in ESRD.Methods: Reference sets of bacteria containing genes of interest were compiled to family, and sets of intestinal bacterial families showing differential abundances between 12 healthy and 24 ESRD individuals enrolled in our original study were compiled. Overlap between sets was assessed using hypergeometric distribution tests.Results: Among 19 microbial families that were dominant in ESRD patients, 12 possessed urease, 5 possessed uricase, and 4 possessed indole and p-cresol-forming enzymes. Among 4 microbial families that were diminished in ESRD patients, 2 possessed butyrate-forming enzymes. Probabilities of these overlapping distributions were <0.05.Conclusions: ESRD patients exhibited significant expansion of bacterial families possessing urease, uricase, and indole and p-cresol forming enzymes, and contraction of families possessing butyrate-forming enzymes. Given the deleterious effects of indoxyl sulfate, p-cresol sulfate, and urea-derived ammonia, and beneficial actions of SCFA, these changes in intestinal microbial metabolism contribute to uremic toxicity and inflammation. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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