Spatial vascular volume fraction imaging for quantitative assessment of angiogenesis.
Purpose: The purpose of this study is to set up a quantitative imaging strategy for therapeutic angiogenesis and monitoring the spatial distribution and survival of the transplanted mesenchymal stem cells (MSCs) in vivo simultaneously.Procedures: Mouse adipose-derived MSCs (AD-MSCs) were isolated fr...
| Publicado en: | Molecular Imaging & Biology Vol. 16; no. 3; pp. 362 - 372 |
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| Autores principales: | , , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Springer Nature
Jun2014
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=103822201&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 103822201 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 15361632 KJU jtl: Molecular Imaging & Biology issn: 15361632 maglogo: N pubinfo: dt: Jun2014 vid: 16 iid: 3 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 103822201 NLM24158404 2012574935 10.1007/s11307-013-0694-z NLM24158404 103822201 ppf: 362 ppct: 10 formats: fmt: @attributes: type: P tig: atl: Spatial vascular volume fraction imaging for quantitative assessment of angiogenesis. aug: au: Liu, Junting Fan, Weiwei Liu, Muhan Lin, Xiuduan Wang, Yabin Wang, Fu Chen, Xiaoyuan Cao, Feng Liang, Jimin affil: School of Life Science and Technology, Xidian University, P.O. Box 97, , No. 2 South Taibai Rd, Xi'an, 710071, China, jiminliang@gmail.com. sug: subj: Neovascularization, Physiologic Lower Extremity Blood Supply Mice Microscopy, Electron, Scanning Tomography, X-Ray Computed Animal Studies ab: Purpose: The purpose of this study is to set up a quantitative imaging strategy for therapeutic angiogenesis and monitoring the spatial distribution and survival of the transplanted mesenchymal stem cells (MSCs) in vivo simultaneously.Procedures: Mouse adipose-derived MSCs (AD-MSCs) were isolated from firefly luciferase and enhanced green fluorescent protein positive transgenic mice, and implanted intramuscularly into hindlimbs of C57BL/6 mice. Serial spatial vascular volume fraction (SVVF) imaging was performed to quantitatively assess angiogenesis by calculating the spatially explicit vascular volume. The hybrid microcomputed tomography angiography/bioluminescence tomography (micro-CTA/BLT) was used to track the fate of AD-MSCs in vivo. Laser Doppler perfusion imaging (LDPI) was used to evaluate blood perfusion. Ex vivo conventional methods were performed to cross-validate the therapeutic angiogenesis.Results: There was a linear correlation relation between the cell number and Fluc/Fluc protein signal intensity in AD-MSCs via BLT. LDPI showed improved perfusion rate in mice treated with cytotherapy, compared to control mice. Furthermore, angiogenesis assessed by SVVF was 10.67 ± 0.41 %, 13.99 ± 0.28 %, and 23.50 ± 1.23 % on days 7, 14, and 28 post-transplantation of AD-MSCs, respectively. Vascular densities of the longitudinally monitored ischemic hindlimbs were significantly higher than those at early time points and controls, which was also confirmed by vascular corrosion casting, scanning electron microscopic imaging, and histological analysis.Conclusions: Hybrid high-resolution micro-CTA/BLT enabled monitoring and quantitative assessment of cytotherapeutically induced angiogenesis in vivo. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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