Five-year incidence of Nd:YAG laser capsulotomy and association with in vitro proliferation of lens epithelial cells from individual specimens: a case control study.
Background: The aims of this study were to determine the 5-year incidence of posterior capsule opacification (PCO) requiring Nd:YAG laser capsulotomy in a representative mixed cohort of cataract patients, to determine risk factors for PCO and to investigate possible association with growth of human...
| Publicado en: | BMC Ophthalmology Vol. 14; no. 1; pp. 116 - 117 |
|---|---|
| Autores principales: | , , , , |
| Formato: | research Journal Article |
| Publicado: |
BioMed Central
2014
|
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=103849209&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 103849209 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 14712415 1CIB jtl: BMC Ophthalmology issn: 14712415 maglogo: N pubinfo: dt: 2014 vid: 14 iid: 1 pid: 24147 pub: BioMed Central artinfo: ui: 103849209 103849209 NLM25274548 2012759402 10.1186/1471-2415-14-116 NLM25274548 PMC4190495 103849209 ppf: 116 ppct: 1 formats: tig: atl: Five-year incidence of Nd:YAG laser capsulotomy and association with in vitro proliferation of lens epithelial cells from individual specimens: a case control study. aug: au: Sundelin, Karin Almarzouki, Nawaf Soltanpour, Yalda Petersen, Anne Zetterberg, Madeleine affil: Institute of Neuroscience and Physiology, Section of Clinical Neuroscience and Rehabilitation/Ophthalmology, The Sahlgrenska Academy at the University of Gothenburg, Sahlgrenska University Hospital, Mölndal SE-431 80, Sweden. madeleine.zetterberg@gu.se. sug: subj: Cataract Epidemiology Cataract Extraction Adverse Effects Epithelial Cells Pathology Laser Therapy Methods Lasers Therapeutic Use Lens, Crystalline Surgery Lenses, Intraocular Aged Cataract Pathology Cell Physiology Cells, Cultured Female Prospective Studies Human Lens, Crystalline Pathology Male Recurrence Retrospective Design Sweden Time Factors Aged: 65+ years Female Male ab: Background: The aims of this study were to determine the 5-year incidence of posterior capsule opacification (PCO) requiring Nd:YAG laser capsulotomy in a representative mixed cohort of cataract patients, to determine risk factors for PCO and to investigate possible association with growth of human lens epithelial cells (HLEC) in vitro.Methods: Pieces of the anterior lens capsule and adhering HLEC were obtained at cataract surgery and cultured individually. After one and two weeks respectively, cultured cells were stained with carboxy-fluorescein diacetate succinimidyl ester (CFDA SE), after which image processing software was used to determine the area of the confluent cell layer. The 5-year incidence of Nd:YAG laser capsulotomy in this cohort was determined through medical records and by mail or telephone interviews. For statistic analyses Mann-Whitney U-test, Fisher's exact test and binary logistic regression were used.Results: Data on treatment/no treatment for PCO was obtained from 270 patients with a median follow-up time of 57 months (range 50-64 months). The three-year cumulative incidence of PCO was 5.2% and the cumulative 5-year incidence was 11.9%. Patients who had undergone Nd:YAG laser capsulotomy were significantly younger (median 71 years) than patients who did not receive treatment for PCO (median 75 years, p = 0.022). Logistic regression demonstrated that apart from younger age, follow-up time and type of intraocular lens (IOL) were associated with risk of PCO, with hydrophilic 1-piece IOLs conferring a higher risk than hydrophobic acrylic 1-piece or 3-piece IOLs (adjusted OR = 9.4, 95% CI 2.5-35.7, p = 0.001). Of the 270 patients from whom information could be retrieved regarding PCO treatment, in vitro cell culture could be established and quantified from 185 patients. No significant difference in cell growth in vitro was shown between patients subsequently requiring/not requiring Nd:YAG laser capsulotomy.Conclusions: The cumulative 5-year incidence of 11.9% is comparable or slightly higher than reported in other recent studies. The type of IOL was the most important risk factor for PCO in this study, whereas intrinsic proliferative capacity of the individual's lens epithelial cells seems to be less important for subsequent PCO development. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|