Quercetin Inhibits Proliferation and Drug Resistance in KB/VCR Oral Cancer Cells and Enhances Its Sensitivity to Vincristine.
Quercetin has been confirmed to possess antihistamine, anti-inflammatory, antiviral, immunomodulatory, and antioxidant properties. Herein, we evaluated their antitumor activity in vitro by using KB/VCR oral cancer cells. We found that quercetin at 25 to 100 μmol/L effectively inhibited the migration...
| Publicado en: | Nutrition & Cancer Vol. 67; no. 1; pp. 126 - 137 |
|---|---|
| Autores principales: | , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Taylor & Francis Ltd
Jan2015
|
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=103866070&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 103866070 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 01635581 7MS jtl: Nutrition & Cancer issn: 01635581 maglogo: N pubinfo: dt: Jan2015 vid: 67 iid: 1 pid: 377 pub: Taylor & Francis Ltd place: Philadelphia, Pennsylvania artinfo: ui: 103866070 100207789 10.1080/01635581.2015.965334 NLM25514492 103866070 ppf: 126 ppct: 11 formats: fmt: @attributes: type: P tig: atl: Quercetin Inhibits Proliferation and Drug Resistance in KB/VCR Oral Cancer Cells and Enhances Its Sensitivity to Vincristine. aug: au: Yuan, Zhaohu Wang, Huili Hu, Ziyou Huang, Yanqing Yao, Fang Sun, Shumei Wu, Bingyi affil: Research Center of Clinical Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, China; Department of Blood Transfusion, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, China sug: subj: Quercetin Therapeutic Use Drug Resistance Mouth Neoplasms Cell Physiology Vincristine Administration and Dosage Nutrition Serial Publications Academic Medical Centers Flavonoids Therapeutic Use In Vitro Studies Cell Culture Techniques Biological Assay DNA Physiology Flow Cytometry Body Surface Area Analysis of Variance Post Hoc Analysis Gene Expression Human ab: Quercetin has been confirmed to possess antihistamine, anti-inflammatory, antiviral, immunomodulatory, and antioxidant properties. Herein, we evaluated their antitumor activity in vitro by using KB/VCR oral cancer cells. We found that quercetin at 25 to 100 μmol/L effectively inhibited the migration and invasion of KB/VCR cells. Quercetin at dose ranging from 25 to 100 μmol/L significantly inhibited the growth of the KB/VCR cells and at 50 μmol/L arrested cells at the G1 phase and decreased the amount of cells in the S and G2 phase. Apoptosis analysis showed that quercetin at 50 or 100 μmol/L induced apoptosis of KB/VCR cells by suppressing expression of Bax and inducing the expression of Caspase-3 and Bcl-2. Furthermore, we also confirmed that quercetin from 25 to 100 μmol/L reversed gene-encoded Pglycoprotein (P-gp)-mediated MDR in KB/VCR cells by inhibiting the expression of P-gp. For combination treatment with vincristin (0.375 μmol/L) and quercetin (50 μmol/L), the proliferation rate significantly decreased and apoptosis rate significantly increased. This study provided evidence that quercetin induced apoptosis and reversed drug resistance in oral tumor cells and may be a potential candidate for other tumors treatment. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|