Detection of Airborne Bacteria in a Duck Production Facility with Two Different Personal Air Sampling Devices for an Exposure Assessment.
Prevalent airborne microorganisms are not well characterized in industrial animal production buildings with respect to their quantity or quality. To investigate the work-related microbial exposure, personal bioaerosol sampling during the whole working day is recommended. Therefore, bioaerosol sampli...
| Publicado en: | Journal of Occupational & Environmental Hygiene Vol. 12; no. 2; pp. 77 - 87 |
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| Autores principales: | , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Taylor & Francis Ltd
Feb2015
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=103872306&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 103872306 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 15459624 V1L jtl: Journal of Occupational & Environmental Hygiene issn: 15459624 maglogo: Y pubinfo: dt: Feb2015 vid: 12 iid: 2 pid: 377 pub: Taylor & Francis Ltd place: Philadelphia, Pennsylvania artinfo: ui: 103872306 100398603 10.1080/15459624.2014.946514 NLM25093856 103872306 ppf: 77 ppct: 10 formats: fmt: @attributes: type: P tig: atl: Detection of Airborne Bacteria in a Duck Production Facility with Two Different Personal Air Sampling Devices for an Exposure Assessment. aug: au: Martin, Elena Dziurowitz, Nico Jäckel, Udo Schäfer, Jenny affil: Federal Institute for Occupational Safety and Health, Berlin, Germany sug: subj: Food Handling Poultry Microbial Contamination Occupational Exposure Evaluation Occupational Exposure Equipment and Supplies In Vitro Studies Germany Air Pollutants, Occupational Aerosols Analysis Microscopy Genetic Techniques Filtration Particle Size Electrophoresis Multimethod Studies ab: Prevalent airborne microorganisms are not well characterized in industrial animal production buildings with respect to their quantity or quality. To investigate the work-related microbial exposure, personal bioaerosol sampling during the whole working day is recommended. Therefore, bioaerosol sampling in a duck hatchery and a duck house with two personal air sampling devices, a filter-based PGP and a NIOSH particle size separator, was performed. Subsequent, quantitative and qualitative analyses were carried out with” culture independent methods. Total cell concentrations (TCC) determined via fluorescence microscopy showed no difference between the two devices. In average, 8 × 106cells/m3were determined in the air of the duck hatchery and 5 × 107cells/m3in the air of the duck house. A Generated Restriction Fragment Length Polymorphism (RFLP) pattern revealed deviant bacterial compositions comparing samples collected with both devices. Clone library analyses based on 16S rRNA gene sequence analysis from the hatchery's air showed 65% similarity between the two sampling devices. Detailed 16S rRNA gene sequence analyses showed the occurrence of bacterial species likeAcinetobacter baumannii, Enterococcus faecalis, Escherichia sp., and Shigella sp.; and a group of Staphylococcus delphini, S. intermedius, and S. pseudintermediusthat provided the evidence of potential exposure to risk group 2 bacteria at the hatchery workplace. Size fractionated sampling with the developed by the Institute for Occupational Safety and Health of the German Social Accident Insurance (IFA) device revealed that pathogenic bacteria would deposit in the inhalable, the thorax, and possibly alveolar dust fraction according to EN481. TCC analysis showed the deposition of bacterial cells in the third stage (< 1μm) at the NIOSH device which implies that bacteria can reach deep into the lungs and contaminate the alveolus after inhalation. Nevertheless, both personal sampling devices could be recommended for exposure assessment at agricultural workplaces. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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