Effect of subinihibitory and inhibitory concentrations of Plectranthus amboinicus (Lour.) Spreng essential oil on Klebsiella pneumoniae.
We evaluated the antimicrobial activity and some mechanisms used by subinhibitory and inhibitory concentrations of the essential oil, obtained from leaves of Plectranthus amboinicus, against a standard strain of Klebsiella pneumoniae and 5 multiresistant clinical isolates of the bacteria. The minima...
| Publicado en: | Phytomedicine Vol. 19; no. 11; pp. 962 - 969 |
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| Autores principales: | , , , , , , , , , , |
| Formato: | Journal Article |
| Publicado: |
Elsevier GmbH, Urban & Fischer Verlag
Aug2012
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=103881870&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 103881870 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 09447113 NXH jtl: Phytomedicine issn: 09447113 maglogo: N pubinfo: dt: Aug2012 vid: 19 iid: 11 pid: 24997 pub: Elsevier GmbH, Urban & Fischer Verlag place: London, <Blank> artinfo: ui: 103881870 2012267764 10.1016/j.phymed.2012.05.013 NLM22776104 103881870 ppf: 962 ppct: 7 formats: tig: atl: Effect of subinihibitory and inhibitory concentrations of Plectranthus amboinicus (Lour.) Spreng essential oil on Klebsiella pneumoniae. aug: au: Gonçalves TB Braga MA de Oliveira FF Santiago GM Carvalho CB Cabral PB Santiago Tde M Sousa JS Barros EB Nascimento RF Nagao-Dias AT affil: Departamento de Análises Clínicas e Toxicológicas, Faculdade de Farmácia, Odontologia e Enfermagem, Universidade Federal Ceará, Rua Capitao Francisco Pedro 1210, CEP 60430-370, Fortaleza, Ceará, Brazil; Curso de Pós-Graduaçao em Microbiologia Médica, Faculdade de Medicina, Universidade Federal do Ceará, Rua Monsenhor Furtado S/N, CEP 60430-270, Fortaleza, Ceará, Brazil. sug: subj: Antibiotics Pharmacodynamics Klebsiella Drug Effects Essential Oils Pharmacodynamics Plant Oils Pharmacodynamics Plants Antibiotics Cell Physiology Drug Effects Drug Resistance, Microbial Hydrogen-Ion Concentration Klebsiella Microbial Culture and Sensitivity Tests Microbiologic Phenomena Drug Effects Essential Oils Plant Leaves Plant Oils Urease Metabolism ab: We evaluated the antimicrobial activity and some mechanisms used by subinhibitory and inhibitory concentrations of the essential oil, obtained from leaves of Plectranthus amboinicus, against a standard strain of Klebsiella pneumoniae and 5 multiresistant clinical isolates of the bacteria. The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC), the rate of kill and the pH sensitivity of the essential oil were determined by microdilution tests performed in 96-well plates. Subinhibitory and inhibitory concentrations of the essential oil were tested in order to check its action on K. pneumoniae membrane permeability, capsule expression, urease activity and cell morphology. The MIC and MBC of the essential oil were 0.09±0.01%. A complete inhibition of the bacterial growth was observed after 2h of incubation with twice the MIC of the essential oil. A better MIC was found when neutral or alkaline pH broth was used. Alteration in membrane permeability was found by the increase of crystal violet uptake when the bacteria were incubated with twice the MIC levels of the essential oil. The urease activity could be prevented when all the subinhibitory concentrations were tested in comparison to the untreated group (p<0.001). Alteration of the bacterial morphology besides inhibition of the capsule expression was verified by atomic force microscopy, and Anthony's stain method, respectively. Our data allow us to conclude that the essential oil of P. amboinicus can be a good candidate for future research. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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