Esophageal-guided biopsy with volumetric laser endomicroscopy and laser cautery marking: a pilot clinical study.

Background: Biopsy surveillance protocols for the assessment of Barrett's esophagus can be subject to sampling errors, resulting in diagnostic uncertainty. Optical coherence tomography is a cross-sectional imaging technique that can be used to conduct volumetric laser endomicroscopy (VLE) of the ent...

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Publicado en:Gastrointestinal Endoscopy Vol. 79; no. 6; pp. 886 - 897
Autores principales: Suter, Melissa J., Gora, Michalina J., Lauwers, Gregory Y., Arnason, Thomas, Sauk, Jenny, Gallagher, Kevin A., Kava, Lauren, Tan, Khay M., Soomro, Amna R., Gallagher, Timothy P., Gardecki, Joseph A., Bouma, Brett E., Rosenberg, Mireille, Nishioka, Norman S., Tearney, Guillermo J.
Formato: clinical trial research tables/charts Journal Article
Publicado: Elsevier B.V. Jun2014
Acceso en línea:Ver este registro en EBSCOhost
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      pub: Elsevier B.V.
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        atl: Esophageal-guided biopsy with volumetric laser endomicroscopy and laser cautery marking: a pilot clinical study.
      aug:
        au:
          Suter, Melissa J.
          Gora, Michalina J.
          Lauwers, Gregory Y.
          Arnason, Thomas
          Sauk, Jenny
          Gallagher, Kevin A.
          Kava, Lauren
          Tan, Khay M.
          Soomro, Amna R.
          Gallagher, Timothy P.
          Gardecki, Joseph A.
          Bouma, Brett E.
          Rosenberg, Mireille
          Nishioka, Norman S.
          Tearney, Guillermo J.
        affil: Pulmonology and Critical Care Unit, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA; Wellman Center for Photomedicine, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA
      sug:
        subj:
          Barrett Esophagus Prognosis
          Endoscopy Methods
          Microscopy Methods
          Biopsy Methods
          Cautery Methods
          Lasers Utilization
          Human
          Academic Medical Centers
          Massachusetts
          Tomography Utilization
          Confidence Intervals Utilization
          Clinical Trials
      ab: Background: Biopsy surveillance protocols for the assessment of Barrett's esophagus can be subject to sampling errors, resulting in diagnostic uncertainty. Optical coherence tomography is a cross-sectional imaging technique that can be used to conduct volumetric laser endomicroscopy (VLE) of the entire distal esophagus. We have developed a biopsy guidance platform that places endoscopically visible marks at VLE-determined biopsy sites. Objective: The objective of this study was to demonstrate in human participants the safety and feasibility of VLE-guided biopsy in vivo. Design: A pilot feasibility study. Setting: Massachusetts General Hospital. Patients: A total of 22 participants were enrolled from January 2011 to June 2012 with a prior diagnosis of Barrett's esophagus. Twelve participants were used to optimize the laser marking parameters and the system platform. A total of 30 target sites were selected and marked in real-time by using the VLE-guided biopsy platform in the remaining 10 participants. Intervention: Volumetric laser endomicroscopy. Main Outcome Measurements: Endoscopic and VLE visibility, and accuracy of VLE diagnosis of the tissue between the laser cautery marks. Results: There were no adverse events of VLE and laser marking. The optimal laser marking parameters were determined to be 2 seconds at 410 mW, with a mark separation of 6 mm. All marks made with these parameters were visible on endoscopy and VLE. The accuracies for diagnosing tissue in between the laser cautery marks by independent blinded readers for endoscopy were 67% (95% confidence interval [CI], 47%-83%), for VLE intent-to-biopsy images 93% (95% CI, 78%-99%), and for corrected VLE post-marking images 100% when compared with histopathology interpretations. Limitations: This is a single-center feasibility study with a limited number of patients. Conclusion: Our results demonstrate that VLE-guided biopsy of the esophagus is safe and can be used to guide biopsy site selection based on the acquired volumetric optical coherence tomography imaging data. (Clinical trial registration number: NCT01439633.)
      pubtype: Academic Journal
      doctype:
        clinical trial
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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