Negative pressure wound therapy is associated with up-regulation of bFGF and ERK1/2 in human diabetic foot wounds.
Chronic foot wounds are a leading cause of morbidity and hospitalization for patients with diabetes. Negative pressure wound therapy ( NPWT) is known to promote healing of diabetic foot wounds, but the underlying molecular mechanisms remain elusive. We propose to gain molecular insights into the wou...
| Publicado en: | Wound Repair & Regeneration Vol. 22; no. 4; pp. 548 - 555 |
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| Autores principales: | , , , , , |
| Formato: | pictorial research tables/charts randomized controlled trial Journal Article |
| Publicado: |
Wiley-Blackwell
Jul/Aug2014
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=103977700&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 103977700 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 10671927 DPV jtl: Wound Repair & Regeneration issn: 10671927 maglogo: Y pubinfo: dt: Jul/Aug2014 vid: 22 iid: 4 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 103977700 97119515 10.1111/wrr.12195 NLM24809625 103977700 ppf: 548 ppct: 7 formats: fmt: @attributes: type: P tig: atl: Negative pressure wound therapy is associated with up-regulation of bFGF and ERK1/2 in human diabetic foot wounds. aug: au: Yang, Shao-Ling Han, Rui Liu, Yu Hu, Li-Ye Li, Xiao-Ling Zhu, Lv-Yun affil: Department of Endocrinology, Peace Hospital of PLA sug: subj: Diabetic Foot Therapy Negative Pressure Wound Therapy Human Randomized Controlled Trials Staining and Labeling Immunohistochemistry Blotting, Western Middle Age Aged Descriptive Statistics Prospective Studies Data Analysis Software One-Way Analysis of Variance Post Hoc Analysis Funding Source Middle Aged: 45-64 years Aged: 65+ years ab: Chronic foot wounds are a leading cause of morbidity and hospitalization for patients with diabetes. Negative pressure wound therapy ( NPWT) is known to promote healing of diabetic foot wounds, but the underlying molecular mechanisms remain elusive. We propose to gain molecular insights into the wound healing promoting signals underlying the effects of NPWT on diabetic foot wounds in humans. We assessed 30 patients with diabetic foot ulcers. Of these cases, 15 were treated with NPWT, while 15 patients were treated with traditional gauze therapy. Granulated tissue was harvested before and after treatment in both patient groups and histologically analyzed with hematoxylin & eosin as well as Masson's trichrome staining methods. Immunohistochemistry and Western blot analysis was performed to evaluate expression of basic fibroblast growth factor ( bFGF) and extracellular signal-regulated kinase ( ERK)1/2, previously associated with promoting cellular growth and/or wound healing. Unlike controls, the wounds in the NPWT-treated diabetic patients developed characteristic features of granulated tissue with increased collagen deposition. Immunohistochemical analysis also revealed an increase in bFGF levels in NPWT-treated patients. Western blot analysis further showed a significant up-regulation of bFGF and phosphorylated ERK1/2 protein levels in the NPWT-treated diabetic patients vs. controls. Our studies reveal that NPWT is associated with an up-regulation of bFGF and ERK1/2 signaling, which may be involved in promoting the NPWT-mediated wound healing response. pubtype: Academic Journal doctype: pictorial research tables/charts randomized controlled trial Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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