Multiple antibiotic-resistant bacteria on fluted pumpkin leaves, a herb of therapeutic value.
Fluted pumpkin (Telfairia occidentalis) is a minimally-processed green leafy vegetable traditionally used for its antianaemic properties in the form of leaf juice without a heating or inactivation step before consumption. The aim of the study was to assess the presence of surface microbiota on T. oc...
| Published in: | Journal of Health, Population & Nutrition Vol. 32; no. 2; pp. 176 - 183 |
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| Main Authors: | , |
| Format: | Journal Article |
| Published: |
BioMed Central
Jun2014
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=103978986&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 103978986 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 16060997 HII jtl: Journal of Health, Population & Nutrition issn: 16060997 maglogo: N pubinfo: dt: Jun2014 vid: 32 iid: 2 pid: 24147 pub: BioMed Central artinfo: ui: 103978986 NLM25076655 2012666764 NLM25076655 103978986 ppf: 176 ppct: 7 formats: fmt: @attributes: type: P tig: atl: Multiple antibiotic-resistant bacteria on fluted pumpkin leaves, a herb of therapeutic value. aug: au: Igbeneghu, Oluwatoyin A Abdu, Abdulrasheed B sug: subj: Antibiotics Pharmacodynamics Bacteria Drug Effects Squash Microbiology Drug Resistance, Microbial Physiology Plant Leaves Microbiology Bacteria Gram-Negative Bacteria Drug Effects Gram-Negative Bacteria Klebsiella Drug Effects Klebsiella Microbial Culture and Sensitivity Tests Methods Proteus Drug Effects Proteus Pseudomonas Drug Effects Pseudomonas Serratia Drug Effects Serratia Staphylococcus Drug Effects Staphylococcus ab: Fluted pumpkin (Telfairia occidentalis) is a minimally-processed green leafy vegetable traditionally used for its antianaemic properties in the form of leaf juice without a heating or inactivation step before consumption. The aim of the study was to assess the presence of surface microbiota on T. occidentalis leaves and also to determine the antimicrobial susceptibility of isolated organisms. Bacterial contaminants on 50 samples of T. occidentalis leaves were isolated and characterized using standard biochemical methods and the antimicrobial susceptibility of isolated organisms was determined using the antibiotic disc diffusion assay. The results obtained show that the leaves of T. occidentalis is contaminated with organisms which included Enterobacter agglomerans (25.9%), Proteus vulgaris (24.9%), Klebsiella spp. (2.6%), and Serratia liquefaciens (2.1%). Other bacterial isolates recovered in order of frequency included: Staphylococcus spp. (33.7%), Bacillus spp. (8.3%), and Pseudomonas fluorescens (2.6%). Of the 193 bacterial isolates from the leaves of T. occidentalis samples tested for antimicrobial resistance, all (100%) were found to be resistant to ampicillin, cloxacillin, augmentin, erythromycin, and tetracycline while 96% of the isolates were resistant to cephalothin. Resistance to trimethoprim (93%) and gentamicin (83%) was also observed. Approximately, 22% of the isolates were resistant to ciprofloxacin; however, only 11 (5.8%) were resistant to ofloxacin. Thus, uncooked T. occidentalis is a potential source of highly-resistant epiphytic bacteria which could be opportunistic pathogens in consumers. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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