Urease inhibitory constituents from Daphne retusa.

The bioassay-guided fractionation ofDaphne retusaHemsl. has led to the isolation of a new aryl tetrahydronaphthalene lignan derivative named as daphnretusic acid (1), along with six new source compounds such as 5,7-dihydroxyflavone (2), 7-hydroxyflavone (3), 6-methoxyflavone (4), (+) pinoresinol (5)...

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Publicado en:Journal of Asian Natural Products Research Vol. 16; no. 2; pp. 210 - 216
Autores principales: Mansoor, Farrukh, Anis, Itrat, Khan, Ajmal, Marasini, Bishnu P., Choudhary, Muhammad Iqbal, Shah, Muhammad Raza
Formato: pictorial research tables/charts Journal Article
Publicado: Taylor & Francis Ltd Feb2014
Acceso en línea:Ver este registro en EBSCOhost
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      jtl: Journal of Asian Natural Products Research
      issn: 10286020
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      dt: Feb2014
      vid: 16
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      pid: 377
      pub: Taylor & Francis Ltd
      place: Philadelphia, Pennsylvania
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        10.1080/10286020.2013.837457
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        atl: Urease inhibitory constituents from Daphne retusa.
      aug:
        au:
          Mansoor, Farrukh
          Anis, Itrat
          Khan, Ajmal
          Marasini, Bishnu P.
          Choudhary, Muhammad Iqbal
          Shah, Muhammad Raza
        affil: Department of Chemistry, University of Karachi, Karachi, 75270, Pakistan
      sug:
        subj:
          Urease Antagonists and Inhibitors
          Plant Extracts Analysis
          Plant Extracts Administration and Dosage
          Lignans Administration and Dosage
          Flavonoids Administration and Dosage
          Peptide Hydrolases Drug Effects
          Molecular Structure
          Magnetic Resonance Spectroscopy
          Mass Spectrometry
          Descriptive Statistics
          Spectrophotometry, Infrared
          Biological Assay
          Chemistry, Pharmaceutical
          Data Analysis Software
          Funding Source
      ab: The bioassay-guided fractionation ofDaphne retusaHemsl. has led to the isolation of a new aryl tetrahydronaphthalene lignan derivative named as daphnretusic acid (1), along with six new source compounds such as 5,7-dihydroxyflavone (2), 7-hydroxyflavone (3), 6-methoxyflavone (4), (+) pinoresinol (5), (+) sesamin (6), and β-sitosterol-3-O-β-D-glucopyranoside (7). Their structures were elucidated by1H NMR,13C NMR, 1D, 2D NMR, UV, IR, and EIMS analyses. All the fractions (n-hexane, CHCl3, AcOEt, CH3OH, and water) and pure compounds (1–7) were subjected to the assay of urease and α-chymotrypsin inhibitory activities. Chloroform and methanol soluble fractions showed moderate urease inhibition. Compound2exhibited significant urease inhibition with IC50value 60.4 ± 0.72 μM, whereas compounds1and3–7remained inactive during urease inhibition and α-chymotrypsin bioassays.
      pubtype: Academic Journal
      doctype:
        pictorial
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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