Age-related changes in the ratio of Mcl-1/Bax expression in the rat cochlea.
Conclusion: Down-regulation of the ratio of Mcl-1/Bax expression may contribute to age-related sensory cell degeneration in the cochlea. Objective: To better understand the involvement of Bcl-2 family members in the regulation of age-related sensory cell death, we examined the expression patterns of...
| Publicado en: | Acta Oto-Laryngologica Vol. 132; no. 2; pp. 123 - 133 |
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| Autores principales: | , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Taylor & Francis Ltd
Feb2012
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104005310&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104005310 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00016489 B6D jtl: Acta Oto-Laryngologica issn: 00016489 maglogo: Y pubinfo: dt: Feb2012 vid: 132 iid: 2 pid: 377 pub: Taylor & Francis Ltd place: Philadelphia, Pennsylvania artinfo: ui: 104005310 93875109 10.3109/00016489.2011.631191 NLM22201517 104005310 ppf: 123 ppct: 10 formats: fmt: @attributes: type: P tig: atl: Age-related changes in the ratio of Mcl-1/Bax expression in the rat cochlea. aug: au: Wei Ping Yang Wei Wei Guo Hui Zhan Liu Yang Xu Lei Chen Bo Hua Hu affil: Institute of Otolaryngology, Chinese PLA General Hospital, Beijing, PR China sug: subj: Gene Expression Evaluation Age Factors Cochlea Hearing Disorders Physiopathology Hair Cells Analysis Animal Studies Rats Reverse Transcriptase Polymerase Chain Reaction Blotting, Western Male Female In Vitro Studies Two-Way Analysis of Variance Descriptive Statistics Data Analysis Software Post Hoc Analysis T-Tests Funding Source Male Female ab: Conclusion: Down-regulation of the ratio of Mcl-1/Bax expression may contribute to age-related sensory cell degeneration in the cochlea. Objective: To better understand the involvement of Bcl-2 family members in the regulation of age-related sensory cell death, we examined the expression patterns of Bcl-2-associated protein X (Bax) and its suppressor, myeloid cell leukemia 1 (Mcl-1) in young and aging rat cochleae. Methods: Young (2-3 months) and aging (27-28 months) Fischer rats were used. The auditory brainstem response (ABR) thresholds elicited by tone bursts at 4, 8, 16, 32, and 40 kHz were measured. The expression patterns of Mcl-1 and Bax genes at both the mRNA and protein levels were examined using a real-time RT-PCR assay and Western blot. Distribution of Mcl-1 and Bax expression in the cochlear sensory epithelia was evaluated using immunohistology and nuclear staining. Results: Aging cochleae exhibited a significant elevation of ABR thresholds. This change was accompanied by significant reduction in Mcl-1 expression at both the mRNA and the protein levels and in the ratio of expression levels of Mcl-1/Bax genes in the aging subjects. Importantly, the changes in Mcl-1 and Bax expression are spatially related to the sensory cells showing the sign of degeneration pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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