Cover Image
QR Code

Engineered Hypopharynx from Coculture of Epithelial Cells and Fibroblasts Using Poly(ester urethane) as Substratum.

Porous polymeric scaffolds have been much investigated and applied in the field of tissue engineering research. Poly(ester urethane) (PEU) scaffolds, comprising pores of 1-20 μm in diameter on one surface and ≥200 μm on the opposite surface and in bulk, were fabricated using phase separation method...

Full description

Published in:BioMed Research International Vol. 2013; pp. 138504 - 138505
Main Authors: Shen, Zhisen, Chen, Jingjing, Kang, Cheng, Gong, Changfeng, Zhu, Yabin
Format: Journal Article
Published: Wiley-Blackwell 2013
Online Access:View this record in EBSCOhost
fields @attributes:
  recordID: 1
pdfLink:
plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104008840&site=ehost-live
header:
  @attributes:
    shortDbName: ccm
    uiTerm: 104008840
    longDbName: CINAHL Complete
    uiTag: AN
  controlInfo:
    bkinfo:
    dissinfo:
    jinfo:
      jid:
        23146133
        FT2T
      jtl: BioMed Research International
      issn: 23146133
      maglogo: N
    pubinfo:
      dt: 2013
      vid: 2013
      pid: 480
      pub: Wiley-Blackwell
      place: Malden, Massachusetts
    artinfo:
      ui:
        104008840
        2012451490
        NLM24455669
        PMC3881389
        104008840
      ppf: 138504
      ppct: 1
      formats:
        fmt:
          @attributes:
            type: P
      tig:
        atl: Engineered Hypopharynx from Coculture of Epithelial Cells and Fibroblasts Using Poly(ester urethane) as Substratum.
      aug:
        au:
          Shen, Zhisen
          Chen, Jingjing
          Kang, Cheng
          Gong, Changfeng
          Zhu, Yabin
        affil: Lihuili Hospital, Ningbo University, Ningbo 315211, China.
      sug:
        subj:
          Epithelial Cells
          Hypopharynx
          Polyesters
          Tissue Engineering
          Biocompatible Materials
          Tissue Culture Techniques
          Fibroblasts
          Human
          Culture Media
      ab: Porous polymeric scaffolds have been much investigated and applied in the field of tissue engineering research. Poly(ester urethane) (PEU) scaffolds, comprising pores of 1-20 μm in diameter on one surface and ≥200 μm on the opposite surface and in bulk, were fabricated using phase separation method for hypopharyngeal tissue engineering. The scaffolds were grafted with silk fibroin (SF) generated from natural silkworm cocoon to enhance the scaffold's hydrophilicity and further improve cytocompatibility to both primary epithelial cells (ECs) and fibroblasts of human hypopharynx tissue. Coculture of ECs and fibroblasts was conducted on the SF-grafted PEU scaffold (PEU-SF) to evaluate its in vitro cytocompatibility. After co-culture for 14 days, ECs were lined on the scaffold surface while fibroblasts were distributed in scaffold bulk. The results of in vivo investigation showed that PEU porous scaffold possessed good biocompatibility after it was grafted by silk fibroin. SF grafting improved the cell/tissue infiltration into scaffold bulk. Thus, PEU-SF porous scaffold is expected to be a good candidate to support the hypopharynx regeneration.
      pubtype: Academic Journal
      doctype: Journal Article
      ougenre: Article
    language: English
    refInfo:
    holdings:
      @attributes:
        islocal: N