[alpha] V [beta] 5 and CD44 Are Oxygen-Regulated Human Embryonic Stem Cell Attachment Factors.
Human embryonic stem cells (hESCs) have great potential for clinical therapeutic use. However, relatively little is known of the mechanisms which dictate their specificity of adhesion to substrates through adhesion proteins including integrins. Previous observations demonstrated enhanced clonogenici...
| Publicado en: | BioMed Research International Vol. 2013; pp. 729281 - 729282 |
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| Autores principales: | , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
2013
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104008880&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104008880 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 2013 vid: 2013 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 104008880 104008880 2012451530 NLM24455718 PMC3888678 104008880 ppf: 729281 ppct: 1 formats: fmt: @attributes: type: P tig: atl: [alpha] V [beta] 5 and CD44 Are Oxygen-Regulated Human Embryonic Stem Cell Attachment Factors. aug: au: Kumar, Deepak Gupta, Saniya Yang, Ying Forsyth, Nicholas R affil: Guy Hilton Research Centre, Institute of Science and Technology in Medicine, University of Keele, Thornburrow Drive, Hartshill, Stoke-on-Trent, Staffordshire ST4 7QB, UK. sug: subj: Antigens, Surface Metabolism Receptors, Cell Surface Metabolism Stem Cells Metabolism Antibodies Immunology Cell Physiology Human Oxygen Metabolism Proteins Metabolism Receptors, Cell Surface Immunology Stem Cells ab: Human embryonic stem cells (hESCs) have great potential for clinical therapeutic use. However, relatively little is known of the mechanisms which dictate their specificity of adhesion to substrates through adhesion proteins including integrins. Previous observations demonstrated enhanced clonogenicity in reduced oxygen culture systems. Here, we demonstrated via antibody blocking experiments that ¿V ß5 and ¿ 6 significantly promoted hESC attachment in 2% O2 only, whereas blockage of CD44 inhibited cell attachment in 21% O2 alone. Immunofluorescence confirmed expression of ¿V ß5 and CD44 in both 2% O2 and 21% O2 cultured hESCs while flow cytometry revealed significantly higher ¿V ß5 expression in 2% O2 versus 21% O2 cultured hESCs and higher CD44 expression in 21% O2 versus 2% O2 cultured hESCs. Adhered hESCs following blockage of ¿V ß5 in 2% O2 displayed a reduction in nuclear colocalisation of Oct-4 and Nanog with little effect observed in 21% O2. Blockage of CD44 had the converse effect with dramatic reductions in nuclear colocalisation of Oct-4 and Nanog in 21% O2 cultured hESC which retained adherence, but not in 2% O2 cultured cells. Identification of oxygen-dependent substrate attachment mechanisms in hESCs has the potential to play a role in the development of novel substrates to improve hESC attachment and culture. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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