Preparative purification of recombinant proteins: current status and future trends.
Advances in fermentation technologies have resulted in the production of increased yields of proteins of economic, biopharmaceutical, and medicinal importance. Consequently, there is an absolute requirement for the development of rapid, cost-effective methodologies which facilitate the purification...
| Publicado en: | BioMed Research International Vol. 2013; pp. 312709 - 312710 |
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| Autores principales: | , , , , , |
| Formato: | Journal Article |
| Publicado: |
Wiley-Blackwell
2013
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104021204&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104021204 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 2013 vid: 2013 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 104021204 2012469975 NLM24455685 PMC3877584 104021204 ppf: 312709 ppct: 1 formats: fmt: @attributes: type: P tig: atl: Preparative purification of recombinant proteins: current status and future trends. aug: au: Saraswat, Mayank Musante, Luca Ravidá, Alessandra Shortt, Brian Byrne, Barry Holthofer, Harry affil: Centre for Bioanalytical Sciences (CBAS), Dublin City University (DCU), Dublin 9, Ireland. sug: subj: Chromatography Methods Recombinant Proteins Chromatography Classification Chromatography Trends Water ab: Advances in fermentation technologies have resulted in the production of increased yields of proteins of economic, biopharmaceutical, and medicinal importance. Consequently, there is an absolute requirement for the development of rapid, cost-effective methodologies which facilitate the purification of such products in the absence of contaminants, such as superfluous proteins and endotoxins. Here, we provide a comprehensive overview of a selection of key purification methodologies currently being applied in both academic and industrial settings and discuss how innovative and effective protocols such as aqueous two-phase partitioning, membrane chromatography, and high-performance tangential flow filtration may be applied independently of or in conjunction with more traditional protocols for downstream processing applications. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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