A Rapid Automatic Processing Platform for Bead Label–Assisted Microarray Analysis: Application for Genetic Hearing-Loss Mutation Detection.
Molecular diagnostics using microarrays are increasingly being used in clinical diagnosis because of their high throughput, sensitivity, and accuracy. However, standard microarray processing takes several hours and involves manual steps during hybridization, slide clean up, and imaging. Here we desc...
| Publicado en: | Journal of Laboratory Automation Vol. 19; no. 2; pp. 144 - 153 |
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| Autores principales: | , , , , , , , , , , |
| Formato: | Journal Article |
| Publicado: |
Sage Publications Inc.
Apr2014
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104048973&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104048973 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 22110682 BG35 jtl: Journal of Laboratory Automation issn: 22110682 maglogo: Y pubinfo: dt: Apr2014 vid: 19 iid: 2 pid: 344 pub: Sage Publications Inc. place: Thousand Oaks, California artinfo: ui: 104048973 2012510504 10.1177/2211068213491096 104048973 ppf: 144 ppct: 9 formats: tig: atl: A Rapid Automatic Processing Platform for Bead Label–Assisted Microarray Analysis: Application for Genetic Hearing-Loss Mutation Detection. aug: au: Zhu, Jiang Song, Xiumei Xiang, Guangxin Feng, Zhengde Guo, Hongju Mei, Danyang Zhang, Guohao Wang, Dong Mitchelson, Keith Xing, Wanli Cheng, Jing affil: CapitalBio Corporation, Beijing, P. R. China, National Engineering Research Center for Beijing Biochip Technology, Beijing, P. R. China sug: subj: Microarray Analysis Biochips Specimen Handling Automation, Laboratory Time Factors Mutation Evaluation Equipment Design Hearing Disorders Familial and Genetic ab: Molecular diagnostics using microarrays are increasingly being used in clinical diagnosis because of their high throughput, sensitivity, and accuracy. However, standard microarray processing takes several hours and involves manual steps during hybridization, slide clean up, and imaging. Here we describe the development of an integrated platform that automates these individual steps as well as significantly shortens the processing time and improves reproducibility. The platform integrates such key elements as a microfluidic chip, flow control system, temperature control system, imaging system, and automated analysis of clinical results. Bead labeling of microarray signals required a simple imaging system and allowed continuous monitoring of the microarray processing. To demonstrate utility, the automated platform was used to genotype hereditary hearing-loss gene mutations. Compared with conventional microarray processing procedures, the platform increases the efficiency and reproducibility of hybridization, speeding microarray processing through to result analysis. The platform also continuously monitors the microarray signals, which can be used to facilitate optimization of microarray processing conditions. In addition, the modular design of the platform lends itself to development of simultaneous processing of multiple microfluidic chips. We believe the novel features of the platform will benefit its use in clinical settings in which fast, low-complexity molecular genetic testing is required. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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