Long-term dietary l-arginine supplementation increases endothelial nitric oxide synthase and vasoactive intestinal peptide immunoexpression in rat small intestine.
Background and aims: Nitric oxide (NO) and vasoactive intestinal polypeptide (VIP) are important intestinal neurotransmitters that coexist in the gut enteric nervous system and play an important role in intestinal physiology (e.g., absorption, motility, fluid secretion and smooth muscle relaxation)....
| Publicado en: | European Journal of Nutrition Vol. 53; no. 3; pp. 813 - 822 |
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| Autores principales: | , , , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Springer Nature
Apr2014
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104058638&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104058638 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 14366207 CR0 jtl: European Journal of Nutrition issn: 14366207 maglogo: N pubinfo: dt: Apr2014 vid: 53 iid: 3 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 104058638 95275544 10.1007/s00394-013-0585-8 NLM24100601 104058638 ppf: 813 ppct: 9 formats: fmt: @attributes: type: P tig: atl: Long-term dietary l-arginine supplementation increases endothelial nitric oxide synthase and vasoactive intestinal peptide immunoexpression in rat small intestine. aug: au: Velickovic, Ksenija Markelic, Milica Golic, Igor Otasevic, Vesna Stancic, Ana Jankovic, Aleksandra Vucetic, Milica Buzadzic, Biljana Korac, Bato Korac, Aleksandra affil: Faculty of Biology, University of Belgrade, Studentski trg 16 11000 Belgrade Serbia sug: subj: Arginine Pharmacodynamics Dietary Supplementation Neuropeptides Metabolism Intestines Metabolism Cold Adaptation, Physiological Oxidoreductases Metabolism Animal Studies In Vivo Studies Funding Source Serbia Rats Descriptive Statistics Immunohistochemistry Histological Techniques Data Analysis Software Comparative Studies Analysis of Variance Post Hoc Analysis Densitometry ab: Background and aims: Nitric oxide (NO) and vasoactive intestinal polypeptide (VIP) are important intestinal neurotransmitters that coexist in the gut enteric nervous system and play an important role in intestinal physiology (e.g., absorption, motility, fluid secretion and smooth muscle relaxation). It is also known that cold exposure alters several aspects of gastrointestinal physiology and induces hyperphagia to meet increased metabolic demands, but there are no data regarding NO and VIP involvement in intestinal response during acclimation to cold. The objective of this study was to determine the influence of long-term l-arginine supplementation on the expression of the three isoforms of nitric oxide synthase (NOS) and VIP in small intestine of rats acclimated to room temperature or cold. Methods: Animals (six per group) acclimated to room temperature (22 ± 1 °C) and cold (4 ± 1 °C), respectively, were treated with 2.25 % l-arginine, a substrate for NOSs, or with 0.01 % N-nitro- l-arginine methyl ester, an inhibitor of NOSs, for 45 days. The topographical distribution of VIP and NOSs expression in small intestine was studied by immunohistochemistry, and ImageJ software was used for semiquantitative densitometric analysis of their immunoexpression. Results: Long-term dietary l-arginine supplementation increases VIP and NOSs immunoexpression at room temperature while at cold increases the endothelial NOS, inducible NOS and VIP but decrease neuronal NOS in rat small intestine. Conclusion: Our results demonstrate that long-term dietary l-arginine supplementation modulates NOSs and VIP immunoexpression in rat small intestine with respect to ambient temperature, pointing out the eNOS as a predominant NOS isoform with an immunoexpression pattern similar to VIP. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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