MRI tracking of macrophages labeled with glucan particles entrapping a water insoluble paramagnetic Gd-based agent.
Purpose: This study is aimed at demonstrating the in vivo potential of Gd(III)-loaded glucan particles (Gd-GPs) as magnetic resonance imaging (MRI)-positive agents for labeling and tracking phagocytic cells.Procedure: GPs were obtained from Saccharomyces cerevisae and loaded with the water-insoluble...
| Publicado en: | Molecular Imaging & Biology Vol. 15; no. 3; pp. 307 - 316 |
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| Autores principales: | , , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Springer Nature
Jun2013
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104072626&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104072626 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 15361632 KJU jtl: Molecular Imaging & Biology issn: 15361632 maglogo: N pubinfo: dt: Jun2013 vid: 15 iid: 3 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 104072626 NLM23179092 2012112268 10.1007/s11307-012-0603-x NLM23179092 104072626 ppf: 307 ppct: 9 formats: fmt: @attributes: type: P tig: atl: MRI tracking of macrophages labeled with glucan particles entrapping a water insoluble paramagnetic Gd-based agent. aug: au: Figueiredo, Sara Cutrin, Juan Carlos Rizzitelli, Silvia De Luca, Elisa Moreira, Joao Nuno Geraldes, Carlos F G C Aime, Silvio Terreno, Enzo Moreira, João Nuno affil: Department of Molecular Biotechnology and Health Sciences and Molecular and Preclinical Imaging Centers, University of Turin, Turin, Italy. sug: subj: Cytological Techniques Methods Elements Diagnostic Use Ferric Compounds Diagnostic Use Glucans Diagnostic Use Macrophages Metabolism Magnetic Resonance Imaging Methods Molecular Imaging Methods Staining and Labeling Animal Studies Cell Line, Tumor Endocytosis Heterocyclic Compounds Diagnostic Use Light Liver Metabolism Male Mice Microscopy Organometallic Compounds Diagnostic Use Rats Solubility Spleen Metabolism Water Yeasts Male ab: Purpose: This study is aimed at demonstrating the in vivo potential of Gd(III)-loaded glucan particles (Gd-GPs) as magnetic resonance imaging (MRI)-positive agents for labeling and tracking phagocytic cells.Procedure: GPs were obtained from Saccharomyces cerevisae and loaded with the water-insoluble complex Gd-DOTAMA(C18)2. The uptake kinetics of Gd-GPs by murine macrophages was studied in vitro and the internalization mechanism was assessed by competition assays. The in vivo performance of Gd-GPs was tested at 7.05 T on a mouse model of acute liver inflammation.Results: The minimum number of Gd-GPs-labeled J774.A1 macrophages detected in vitro by MRI was ca. 300 cells/μl of agar, which is the lowest number ever reported for cells labeled with a positive T1 agent. Intravenous injection of macrophages labeled with Gd-GPs in a mouse model of liver inflammation enabled the MRI visualization of the cellular infiltration in the diseased area.Conclusions: Gd-GPs represent a promising platform for tracking macrophages by MRI as a T1 alternative to the golden standard T2-based iron oxide particles. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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