Preparation, modification, and characterization of alginate hydrogel with nano-/microfibers: a new perspective for tissue engineering.
We aimed to develop an alginate hydrogel (AH) modified with nano-/microfibers of titanium dioxide (nfTD) and hydroxyapatite (nfHY) and evaluated its biological and chemical properties. Nano-/microfibers of nfTD and nfHY were combined with AH, and its chemical properties were evaluated by FTIR spectr...
| Publicado en: | BioMed Research International Vol. 2013; pp. 307602 - 307603 |
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| Autores principales: | , , , , , |
| Formato: | Journal Article |
| Publicado: |
Wiley-Blackwell
2013
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104082044&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104082044 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 2013 vid: 2013 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 104082044 2012190179 NLM23862142 PMC3687604 104082044 ppf: 307602 ppct: 1 formats: fmt: @attributes: type: P tig: atl: Preparation, modification, and characterization of alginate hydrogel with nano-/microfibers: a new perspective for tissue engineering. aug: au: Palma Santana, Bianca Nedel, Fernanda Piva, Evandro Varella de Carvalho, Rodrigo Fernando Demarco, Flávio Lenin Villarreal Carreño, Neftali affil: Nucleus of Cellular and Tecidual Biology (NCTBio), Post-Graduate Program in Dentistry, Federal University of Pelotas, Rua Gonçalves Chaves 457, Centro, 96015-560 Pelotas, RS, Brazil. sug: subj: Alginates Gels Technology Tissue Engineering Methods Alginates Pharmacodynamics Animals Cell Death Drug Effects Cell Physiology Drug Effects Minerals Pharmacodynamics Fibroblasts Fibroblasts Drug Effects Acids, Acyclic Acids, Acyclic Pharmacodynamics Gels Pharmacodynamics Materials Testing Mice Cells Spectrometry, X-Ray Emission Spectrophotometry, Infrared Titanium Pharmacodynamics X-Rays ab: We aimed to develop an alginate hydrogel (AH) modified with nano-/microfibers of titanium dioxide (nfTD) and hydroxyapatite (nfHY) and evaluated its biological and chemical properties. Nano-/microfibers of nfTD and nfHY were combined with AH, and its chemical properties were evaluated by FTIR spectroscopy, X-ray diffraction, energy dispersive X-Ray analysis, and the cytocompatibility by the WST-1 assay. The results demonstrate that the association of nfTD and nfHY nano-/microfibers to AH did not modified the chemical characteristics of the scaffold and that the association was not cytotoxic. In the first 3 h of culture with NIH/3T3 cells nfHY AH scaffolds showed a slight increase in cell viability when compared to AH alone or associated with nfTD. However, an increase in cell viability was observed in 24 h when nfTD was associated with AH scaffold. In conclusion our study demonstrates that the combination of nfHY and nfTD nano-/microfibers in AH scaffold maintains the chemical characteristics of alginate and that this association is cytocompatible. Additionally the combination of nfHY with AH favored cell viability in a short term, and the addition of nfTD increased cell viability in a long term. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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