Molecular Characterization of BK and JC Viruses Circulating among Potential Kidney Donors in Kuwait.

BK and JC polyomaviruses can be associated with nephropathy following renal transplantation. The aim of this study was to determine the prevalence, load, and genotypes of BK and JC viruses circulated in potential kidney donors in Kuwait. The detection of polyomavirus DNA was carried out in serum and...

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Publicado en:BioMed Research International Vol. 2013; pp. 683464 - 683465
Autores principales: Chehadeh, Wassim, Kurien, Susan Silpi, Nampoory, Mangalathillam Raman
Formato: research Journal Article
Publicado: Wiley-Blackwell 2013
Acceso en línea:Ver este registro en EBSCOhost
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      dt: 2013
      vid: 2013
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      pub: Wiley-Blackwell
      place: Malden, Massachusetts
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        atl: Molecular Characterization of BK and JC Viruses Circulating among Potential Kidney Donors in Kuwait.
      aug:
        au:
          Chehadeh, Wassim
          Kurien, Susan Silpi
          Nampoory, Mangalathillam Raman
        affil: Virology Unit, Department of Microbiology, Faculty of Medicine, Kuwait University, P.O. Box 24923, Safat, Jabriya 13310, Kuwait.
      sug:
        subj:
          Viruses
          Kidney Transplantation Adverse Effects
          Transplant Donors
          Adult
          Aged
          Nucleotides
          DNA Blood
          DNA Urine
          Female
          Human
          Kuwait
          Male
          Middle Age
          Adult: 19-44 years
          Aged: 65+ years
          Middle Aged: 45-64 years
          Female
          Male
      ab: BK and JC polyomaviruses can be associated with nephropathy following renal transplantation. The aim of this study was to determine the prevalence, load, and genotypes of BK and JC viruses circulated in potential kidney donors in Kuwait. The detection of polyomavirus DNA was carried out in serum and urine samples of 165 potential kidney donors. Seventy (42%) individuals were tested positive for polyomavirus DNA, of whom 20 (12%) had detectable polyomavirus DNA in their serum samples, 40 (24%) in their urine samples, and 10 (6%) in both serum and urine samples. In the group of polyomavirus-positive patients, JC DNA could be detected in 78% of urine samples and 11% of serum samples, whereas BK DNA could be detected in 7% of urine samples and 3% of serum samples. The median polyomaviral load was low. The detected BK sequences in Kuwaiti adults formed new clusters sharing common ancestor with subgroups Ib1 and IVc, which are prevalent in Asia and Europe. Additionally, around half of the detected JCV sequences in Kuwaiti adults formed new clusters within the African subtype 3. Our results suggest high rate of polyomavirus shedding among healthy adults in Kuwait that can jeopardize their suitability for kidney donation.
      pubtype: Academic Journal
      doctype:
        research
        Journal Article
      ougenre: Article
    language: English
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