Modulation of Vasodilator Response via the Nitric Oxide Pathway after Acute Methyl Mercury Chloride Exposure in Rats.

Mercury exposure induces endothelial dysfunction leading to loss of endothelium-dependent vasorelaxation due to decreased nitric oxide (NO) bioavailability via increased oxidative stress. Our aim was to investigate whether acute treatment with methyl mercury chloride changes the endothelium-dependen...

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Publicado en:BioMed Research International Vol. 2013; pp. 530603 - 530604
Autores principales: Omanwar, S, Saidullah, B, Ravi, K, Fahim, M
Formato: Journal Article
Publicado: Wiley-Blackwell 2013
Acceso en línea:Ver este registro en EBSCOhost
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      dt: 2013
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      pub: Wiley-Blackwell
      place: Malden, Massachusetts
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        atl: Modulation of Vasodilator Response via the Nitric Oxide Pathway after Acute Methyl Mercury Chloride Exposure in Rats.
      aug:
        au:
          Omanwar, S
          Saidullah, B
          Ravi, K
          Fahim, M
        affil: School of Sciences, Indira Gandhi National Open University, New Delhi 110068, India.
      sug:
        subj:
          Acetylcholine Administration and Dosage
          Organic Chemicals Administration and Dosage
          Nitric Oxide Metabolism
          Vasodilator Agents Administration and Dosage
          Animals
          Biological Availability
          Biological Factors Metabolism
          Lipid Peroxidation Drug Effects
          Malondialdehyde Metabolism
          Metabolic Networks and Pathways Drug Effects
          Oxidoreductases Metabolism
          Tissue Culture Techniques
          Oxidative Stress Drug Effects
          Rats
          Vasodilation Drug Effects
      ab: Mercury exposure induces endothelial dysfunction leading to loss of endothelium-dependent vasorelaxation due to decreased nitric oxide (NO) bioavailability via increased oxidative stress. Our aim was to investigate whether acute treatment with methyl mercury chloride changes the endothelium-dependent vasodilator response and to explore the possible mechanisms behind the observed effects. Wistar rats were treated with methyl mercury chloride (5 mg/kg, po.). The methyl mercury chloride treatment resulted in an increased aortic vasorelaxant response to acetylcholine (ACh). In methyl-mercury-chloride-exposed rats, the % change in vasorelaxant response of ACh in presence of Nw-Nitro-L-arginine methyl ester hydrochloride (L-NAME; 10-4 M) was significantly increased, and in presence of glybenclamide (10-5 M), the response was similar to that of untreated rats, indicating the involvement of NO and not of endothelium-derived hyperpolarizing factor (EDHF). In addition, superoxide dismutase (SOD) + catalase treatment increased the NO modulation of vasodilator response in methyl-mercury-chloride-exposed rats. Our results demonstrate an increase in the vascular reactivity to ACh in aorta of rats acutely exposed to methyl mercury chloride. Methyl mercury chloride induces nitric oxide synthase (NOS) and increases the NO production along with inducing oxidative stress without affecting the EDHF pathway.
      pubtype: Academic Journal
      doctype: Journal Article
      ougenre: Article
    language: English
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