Inflammatory bowel disease therapies and gut function in a colitis mouse model.
Background. Exclusive enteral nutrition (EEN) is a well-established approach to the management of Crohn's disease. Aim. To determine effects of EEN upon inflammation and gut barrier function in a colitis mouse model. Methods. Interleukin-10-deficient mice were inoculated with Helicobacter trogontum...
| Publicado en: | BioMed Research International Vol. 2013; pp. 909613 - 909614 |
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| Autores principales: | , , , , , , , |
| Formato: | Journal Article |
| Publicado: |
Wiley-Blackwell
2013
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104094730&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104094730 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 2013 vid: 2013 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 104094730 2012259412 NLM24027765 PMC3763566 104094730 ppf: 909613 ppct: 1 formats: fmt: @attributes: type: P tig: atl: Inflammatory bowel disease therapies and gut function in a colitis mouse model. aug: au: Nahidi, Lily Leach, Steven T Mitchell, Hazel M Kaakoush, Nadeem O Lemberg, Daniel A Munday, John S Huinao, Karina Day, Andrew S affil: School of Women's and Children's Health, University of New South Wales, Randwick, Sydney, NSW 2031, Australia. sug: subj: Colitis Pathology Crohn Disease Pathology Inflammatory Bowel Diseases Pathology Interleukins Animals Colitis Microbiology Colitis Therapy Crohn Disease Microbiology Crohn Disease Therapy Models, Biological Enteral Nutrition Helicobacter Inflammation Microbiology Inflammation Pathology Inflammatory Bowel Diseases Microbiology Inflammatory Bowel Diseases Therapy Interleukins Metabolism Intestinal Mucosa Drug Effects Intestinal Mucosa Metabolism Metronidazole Administration and Dosage Mice RNA Metabolism ab: Background. Exclusive enteral nutrition (EEN) is a well-established approach to the management of Crohn's disease. Aim. To determine effects of EEN upon inflammation and gut barrier function in a colitis mouse model. Methods. Interleukin-10-deficient mice were inoculated with Helicobacter trogontum and then treated with EEN, metronidazole, hydrocortisone, or EEN and metronidazole combination. Blood and tissue were collected at 2 and 4 weeks with histology, mucosal integrity, tight junction integrity, inflammation, and H. trogontum load evaluated. Results. H. trogontum induced colitis in IL-10-/- mice with histological changes in the cecum and colon. Elevated mucosal IL-8 mRNA in infected mice was associated with intestinal barrier dysfunction indicated by decreased transepithelial electrical resistance and mRNA of tight junction proteins and increased short-circuit current, myosin light chain kinase mRNA, paracellular permeability, and tumor necrosis factor-a and myeloperoxidase plasma levels (P < 0.01 for all comparisons). EEN and metronidazole, but not hydrocortisone, treatments restored barrier function, maintained gut barrier integrity, and reversed inflammatory changes along with reduction of H. trogontum load (versus infected controls P < 0.05). Conclusion. H. trogontum infection in IL-10-/- mice induced typhlocolitis with intestinal barrier dysfunction. EEN and metronidazole, but not hydrocortisone, modulate barrier dysfunction and reversal of inflammatory changes. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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