Synchrotron radiation X-ray phase micro-computed tomography as a new method to detect iron oxide nanoparticles in the brain.
Purpose: The purpose of this study was to introduce synchrotron radiation X-ray phase computed tomography (SR-PCT) as a new method of visualizing ultrasmall superparamagnetic particles of iron oxide (USPIO) distribution into the brains of mice with neuroinflammation.Procedures: The sensitivity of th...
| Publicado en: | Molecular Imaging & Biology Vol. 15; no. 5; pp. 552 - 560 |
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| Autores principales: | , , , , , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Springer Nature
Oct2013
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104098018&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104098018 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 15361632 KJU jtl: Molecular Imaging & Biology issn: 15361632 maglogo: N pubinfo: dt: Oct2013 vid: 15 iid: 5 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 104098018 NLM23632952 2012308145 10.1007/s11307-013-0639-6 NLM23632952 104098018 ppf: 552 ppct: 8 formats: fmt: @attributes: type: P tig: atl: Synchrotron radiation X-ray phase micro-computed tomography as a new method to detect iron oxide nanoparticles in the brain. aug: au: Marinescu, M Langer, M Durand, A Olivier, C Chabrol, A Rositi, H Chauveau, F Cho, T H Nighoghossian, N Berthezène, Y Peyrin, F Wiart, M affil: Université de Lyon, Lyon 1, France. sug: subj: Brain Metabolism Dextrans Metabolism Particle Accelerators Tomography, X-Ray Computed Methods Animal Studies Brain Pathology Cerebral Ischemia Diagnosis Cerebral Ischemia Pathology Ferric Compounds Image Processing, Computer Assisted Magnetic Resonance Imaging Male Mice Models, Biological Phantoms, Imaging Refractometry Stereotaxic Techniques Male ab: Purpose: The purpose of this study was to introduce synchrotron radiation X-ray phase computed tomography (SR-PCT) as a new method of visualizing ultrasmall superparamagnetic particles of iron oxide (USPIO) distribution into the brains of mice with neuroinflammation.Procedures: The sensitivity of the technique was assessed by performing back-to-back SR-PCT and magnetic resonance imaging (MRI) in mice stereotaxically injected with a range of USPIO concentrations. Eight mice with cerebral ischemia were then intravenously injected with USPIOs and imaged back-to-back with MRI and SR-PCT.Results: SR-PCT proved sensitive enough to detect iron in nanomolar quantities. In stroke-induced animals, SR-PCT showed hyperintense areas in the regions of MR signal loss and immunostaining for macrophages. SR-PCT, moreover, identified brain anatomy as clearly as histology, without the need for sectioning or staining, with an examination time of 44 min per brain at an isotropic spatial resolution of 8 μm.Conclusion: SR-PCT has potential for cellular imaging in intact brain, with unequaled neuroanatomy. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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