Effects of gamma irradiation on bacterial microflora associated with human amniotic membrane.
Human amniotic membrane is considered a promising allograft material for the treatment of ocular surface reconstruction, burns, and other skin defects. In order to avoid the transmission of any diseases, grafts should be perfectly sterile. Twenty-five amniotic sacs were collected to determine the mi...
| Published in: | BioMed Research International Vol. 2013; pp. 586561 - 586562 |
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| Main Authors: | , , , |
| Format: | research Journal Article |
| Published: |
Wiley-Blackwell
2013
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104098174&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104098174 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 2013 vid: 2013 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 104098174 104098174 2012308486 NLM24063009 PMC3770025 104098174 ppf: 586561 ppct: 1 formats: fmt: @attributes: type: P tig: atl: Effects of gamma irradiation on bacterial microflora associated with human amniotic membrane. aug: au: Binte Atique, Fahmida Ahmed, Kazi Tahsin Asaduzzaman, S M Hasan, Kazi Nadim affil: Department of Biochemistry and Microbiology, School of Life Sciences, North South University, Bangladesh. sug: subj: Amnion Microbiology Amnion Radiation Effects Bacteria Radiation Effects gamma Rays Bacteria Human Microbiologic Phenomena Radiation Effects Radiation Dosage Sterilization and Disinfection ab: Human amniotic membrane is considered a promising allograft material for the treatment of ocular surface reconstruction, burns, and other skin defects. In order to avoid the transmission of any diseases, grafts should be perfectly sterile. Twenty-five amniotic sacs were collected to determine the microbiological quality ofhuman amniotic membrane, to analyze the radiation sensitivity pattern of the microorganism, and to detect the radiation decimal reduction dose (D10) values. All the samples were found to be contaminated, and the bioburden was ranged from 3.4 x 10² to 1.2 x 105cfu/g. Initially, a total fifty bacterial isolates were characterized according to their cultural, morphological, and biochemical characteristics and then tested for the radiation sensitivity in an incremental series of radiation doses from 1 to 10 KGy. The results depict gradual decline in bioburden with incline of radiation doses. Staphylococcus spp. were the most frequently isolated bacterial contaminant in tissue samples (44%). The D10 values of the bacterial isolates were ranged from 0.6 to 1.27 KGy. Streptococcus spp. were found to be the highest radioresistant strain with the radiation sterilization dose (RSD) of 11.4 KGy for a bioburden level of1000. To compare the differences, D10 values were also calculated by graphical evaluations of the data with two of the representative isolates of each bacterial species which showed no significant variations. Findings of this study indicate that lower radiation dose is quite satisfactory for the sterilization of amniotic membrane grafts. Therefore, these findings would be helpful to predict the efficacy of radiation doses for the processing of amniotic membrane for various purposes. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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