Digital microscopy assessment of angiogenesis in different breast cancer compartments.
Background/Aim. Tumour angiogenesis defined by microvessel density (MVD) is generally accepted as a prognostic factor in breast cancer. However, due to variability of measurement systems and cutoffs, it is questionable to date whether it contributes to predictive outline. Our study aims to grade vas...
| Published in: | BioMed Research International Vol. 2013; pp. 286902 - 286903 |
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| Main Authors: | , , , , , , |
| Format: | Journal Article |
| Published: |
Wiley-Blackwell
2013
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104098880&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104098880 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 2013 vid: 2013 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 104098880 2012313461 NLM24073397 PMC3773887 104098880 ppf: 286902 ppct: 1 formats: fmt: @attributes: type: P tig: atl: Digital microscopy assessment of angiogenesis in different breast cancer compartments. aug: au: Haisan, Anca Rogojanu, Radu Croitoru, Camelia Jitaru, Daniela Tarniceriu, Cristina Danciu, Mihai Carasevici, Eugen affil: Emergency Medicine Department, Gr. T. Popa University of Medicine and Pharmacy, 700115 Iasi, Romania ; Emergency Medicine Department, Saint Spiridon Hospital, 700115 Iasi, Romania. sug: subj: Breast Neoplasms Blood Supply Breast Neoplasms Pathology Microscopy Methods Neovascularization, Pathologic Pathology Adult Aged Antigens, Surface Metabolism Endothelium Pathology Female Middle Age Neoplasm Invasiveness Statistics Connective Tissue Cells Pathology Adult: 19-44 years Aged: 65+ years Middle Aged: 45-64 years Female ab: Background/Aim. Tumour angiogenesis defined by microvessel density (MVD) is generally accepted as a prognostic factor in breast cancer. However, due to variability of measurement systems and cutoffs, it is questionable to date whether it contributes to predictive outline. Our study aims to grade vascular heterogeneity by comparing clear-cut compartments: tumour associated stroma (TAS), tumour parenchyma, and tumour invasive front. Material and Methods. Computerized vessel area measurement was performed using a tissue cytometry system (TissueFAXS) on slides originated from 50 patients with breast cancer. Vessels were marked using immunohistochemistry with CD34. Regions of interest were manually defined for each tumour compartment. Results. Tumour invasive front vascular endothelia area was 2.15 times higher than that in tumour parenchyma and 4.61 times higher than that in TAS (P < 0.002). Worth to mention that the lymph node negative subgroup of patients show a slight but constant increase of vessel index in all examined compartments of breast tumour. Conclusion. Whole slide digital examination and region of interest (ROI) analysis are a valuable tool in scoring angiogenesis markers and disclosing their prognostic capacity. Our study reveals compartments' variability of vessel density inside the tumour and highlights the propensity of invasive front to associate an active process of angiogenesis with potential implications in adjuvant therapy. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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