Prokaryotic phylogenies inferred from whole-genome sequence and annotation data.

Phylogenetic trees are used to represent the evolutionary relationship among various groups of species. In this paper, a novel method for inferring prokaryotic phylogenies using multiple genomic information is proposed. The method is called CGCPhy and based on the distance matrix of orthologous gene...

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Publicado en:BioMed Research International Vol. 2013; pp. 409062 - 409063
Autores principales: Du, Wei, Cao, Zhongbo, Wang, Yan, Sun, Ying, Blanzieri, Enrico, Liang, Yanchun
Formato: Journal Article
Publicado: Wiley-Blackwell 2013
Acceso en línea:Ver este registro en EBSCOhost
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      dt: 2013
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      pub: Wiley-Blackwell
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        atl: Prokaryotic phylogenies inferred from whole-genome sequence and annotation data.
      aug:
        au:
          Du, Wei
          Cao, Zhongbo
          Wang, Yan
          Sun, Ying
          Blanzieri, Enrico
          Liang, Yanchun
        affil: Key Laboratory of Symbol Computation and Knowledge Engineering of the Ministry of Education, College of Computer Science and Technology, Jilin University, Changchun 130012, China ; College of Chemistry, Jilin University, Changchun 130012, China.
      sug:
        subj:
          Resource Databases
          Genome
          Sequence Analysis
          Evolution
          Cells Metabolism
          Molecular Structure
          Genes
          Reproducibility of Results
      ab: Phylogenetic trees are used to represent the evolutionary relationship among various groups of species. In this paper, a novel method for inferring prokaryotic phylogenies using multiple genomic information is proposed. The method is called CGCPhy and based on the distance matrix of orthologous gene clusters between whole-genome pairs. CGCPhy comprises four main steps. First, orthologous genes are determined by sequence similarity, genomic function, and genomic structure information. Second, genes involving potential HGT events are eliminated, since such genes are considered to be the highly conserved genes across different species and the genes located on fragments with abnormal genome barcode. Third, we calculate the distance of the orthologous gene clusters between each genome pair in terms of the number of orthologous genes in conserved clusters. Finally, the neighbor-joining method is employed to construct phylogenetic trees across different species. CGCPhy has been examined on different datasets from 617 complete single-chromosome prokaryotic genomes and achieved applicative accuracies on different species sets in agreement with Bergey's taxonomy in quartet topologies. Simulation results show that CGCPhy achieves high average accuracy and has a low standard deviation on different datasets, so it has an applicative potential for phylogenetic analysis.
      pubtype: Academic Journal
      doctype: Journal Article
      ougenre: Article
    language: English
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