Improved Properties of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Produced by Comamonas sp. EB172 Utilizing Volatile Fatty Acids by Regulating the Nitrogen Source.

This study presents the effect of carbon to nitrogen ratio (C/N) (mol/mol) on the cell growth and poly(3-hydroxybutyrate-co-3- hydroxyvalerate) accumulation by Comamonas sp. EB172 in 2 L fermenters using volatile fatty acids (VFA) as the carbon source. This VFA was supplemented with ammonium sulphat...

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Publicado en:BioMed Research International Vol. 2013; pp. 237806 - 237807
Autores principales: Zakaria, Mohd Rafein, Ariffin, Hidayah, Abd-Aziz, Suraini, Hassan, Mohd Ali, Shirai, Yoshihito
Formato: research Journal Article
Publicado: Wiley-Blackwell 2013
Acceso en línea:Ver este registro en EBSCOhost
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      dt: 2013
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      pub: Wiley-Blackwell
      place: Malden, Massachusetts
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        atl: Improved Properties of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Produced by Comamonas sp. EB172 Utilizing Volatile Fatty Acids by Regulating the Nitrogen Source.
      aug:
        au:
          Zakaria, Mohd Rafein
          Ariffin, Hidayah
          Abd-Aziz, Suraini
          Hassan, Mohd Ali
          Shirai, Yoshihito
        affil: Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
      sug:
        subj:
          Carbon
          Fatty Acids, Volatile
          Polyesters
          Polymers
          Gram-Negative Aerobic Bacteria
          Gram-Negative Aerobic Bacteria Metabolism
          Fermentation
          Chemistry, Physical
          Nitrogen
      ab: This study presents the effect of carbon to nitrogen ratio (C/N) (mol/mol) on the cell growth and poly(3-hydroxybutyrate-co-3- hydroxyvalerate) accumulation by Comamonas sp. EB172 in 2 L fermenters using volatile fatty acids (VFA) as the carbon source. This VFA was supplemented with ammonium sulphate and yeast extract in the feeding solution to achieve C/N (mol/mol) 5,15,25, and 34.4, respectively. By extrapolating the C/N and the source of nitrogen, the properties of the polymers can be regulated. The number average molecular weight (Mn) of P(3HB-co-3HV) copolymer reached the highest at 838 x 10³ Da with polydispersity index (PDI) value of 1.8, when the culture broth was supplemented with yeast extract (C/N 34.4). Tensile strength and Young's modulus of the copolymer containing 6 - 8 mol% 3HV were in the ranges of 13-14.4 MPa and 0.26-0.34 GPa, respectively, comparable to those of polyethylene (PE). Thus, Comamonas sp. EB172 has shown promising bacterial isolates producing polyhydroxyalkanoates from renewable carbon materials.
      pubtype: Academic Journal
      doctype:
        research
        Journal Article
      ougenre: Article
    language: English
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