Allergy or tolerance: reduced inflammatory cytokine response and concomitant IL-10 production of lymphocytes and monocytes in symptom-free titanium dental implant patients.

Hypersensitivity reactions to titanium (Ti) are very rare. Thus, we assessed the proinflammatory response and also potential tolerance favoring in vitro reactivity ofhuman blood lymphocytes and monocytes (PBMC) to Ti in healthy individuals (14 without, 6 with complication-free dental Ti implants). T...

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Publicado en:BioMed Research International Vol. 2013; pp. 539834 - 539835
Autores principales: Thomas, Peter, Iglhaut, Gerhard, Wollenberg, Andreas, Cadosch, Dieter, Summer, Burkhard
Formato: Journal Article
Publicado: Wiley-Blackwell 2013
Acceso en línea:Ver este registro en EBSCOhost
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      dt: 2013
      vid: 2013
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      pub: Wiley-Blackwell
      place: Malden, Massachusetts
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        atl: Allergy or tolerance: reduced inflammatory cytokine response and concomitant IL-10 production of lymphocytes and monocytes in symptom-free titanium dental implant patients.
      aug:
        au:
          Thomas, Peter
          Iglhaut, Gerhard
          Wollenberg, Andreas
          Cadosch, Dieter
          Summer, Burkhard
        affil: Department of Dermatology and Allergology, Ludwig-Maximilians-University of Munich, Frauenlobstraße 9-11, 80337 Munich, Germany.
      sug:
        subj:
          Dental Implants Adverse Effects
          Interleukins
          Titanium Adverse Effects
          Adult
          Cells, Cultured Drug Effects
          Cells, Cultured Metabolism
          Cytokines
          Female
          Hypersensitivity Metabolism
          Hypersensitivity Pathology
          Immunity
          Interleukins Metabolism
          Lymphocytes Immunology
          Lymphocytes Metabolism
          Lymphocytes Pathology
          Male
          Middle Age
          Monocytes Immunology
          Monocytes Metabolism
          Monocytes Pathology
          Titanium Administration and Dosage
          Titanium Therapeutic Use
          Adult: 19-44 years
          Middle Aged: 45-64 years
          Female
          Male
      ab: Hypersensitivity reactions to titanium (Ti) are very rare. Thus, we assessed the proinflammatory response and also potential tolerance favoring in vitro reactivity ofhuman blood lymphocytes and monocytes (PBMC) to Ti in healthy individuals (14 without, 6 with complication-free dental Ti implants). The proliferation index (SI) in lymphocyte transformation test (LTT) and production of cytokines linked to innate immune response (IL-1β, IL-6, and TNFα) or immune regulation (IL-10) were assessed in response to TiO2 particles or Ti discs. In both groups, the Ti-LTT reactivity was not enhanced (e.g., SI < 3). The control antigen tetanus toxoid (TT) gave adequate reactivity (median SI individuals without/with implant: 20.6 ± 5.97/19.58 ± 2.99). Individuals without implant showed higher cytokine response to Ti materials than individuals with symptom-free implants; for example, TiO2 rutile particle induced increase of IL-1β 70.27-fold/8.49-fold versus control medium culture. PBMC of 5 of the 6 individuals with complicationfree Ti implants showed an ex vivo ongoing production of IL-10 (mean 4.18 ± 2.98 pg/mL)-but none of the 14 controls showed such IL-10 production. Thus in vitro IL-1β-, IL-6-, and TNF-α production reflects "normal" unspecific immune response to Ti. This might be reduced by production of tolerogenic IL-10 in individuals with symptom-free Ti dental implants.
      pubtype: Academic Journal
      doctype: Journal Article
      ougenre: Article
    language: English
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