Biomimetic mineralization on a macroporous cellulose-based matrix for bone regeneration.

The aim of this study is to investigate the biomimetic mineralization on a cellulose-based porous matrix with an improved biological profile. The cellulose matrix was precalcified using three methods: (i) cellulose samples were treated with a solution of calcium chloride and diammonium hydrogen phos...

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Publicado en:BioMed Research International Vol. 2013; pp. 452750 - 452751
Autores principales: Petrauskaite, Odeta, Gomes, Pedro de Sousa, Fernandes, Maria Helena, Juodzbalys, Gintaras, Stumbras, Arturas, Maminskas, Julius, Liesiene, Jolanta, Cicciù, Marco
Formato: research Journal Article
Publicado: Wiley-Blackwell 2013
Acceso en línea:Ver este registro en EBSCOhost
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      dt: 2013
      vid: 2013
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      pub: Wiley-Blackwell
      place: Malden, Massachusetts
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        atl: Biomimetic mineralization on a macroporous cellulose-based matrix for bone regeneration.
      aug:
        au:
          Petrauskaite, Odeta
          Gomes, Pedro de Sousa
          Fernandes, Maria Helena
          Juodzbalys, Gintaras
          Stumbras, Arturas
          Maminskas, Julius
          Liesiene, Jolanta
          Cicciù, Marco
        affil: Department of Organic Technology, Kaunas University of Technology, Radvilenu pl. 19, 50254 Kaunas, Lithuania.
      sug:
        subj:
          Industry
          Bone Regeneration Drug Effects
          Cellulose
          Connective Tissue Cells
          Bone Development Drug Effects
          Calcium Chloride Pharmacodynamics
          Cell Physiology Drug Effects
          Cellulose Metabolism
          Minerals
          Minerals Therapeutic Use
          Human
          Connective Tissue Cells Drug Effects
          Connective Tissue Cells Metabolism
          Phosphates Pharmacodynamics
          Surface Properties
      ab: The aim of this study is to investigate the biomimetic mineralization on a cellulose-based porous matrix with an improved biological profile. The cellulose matrix was precalcified using three methods: (i) cellulose samples were treated with a solution of calcium chloride and diammonium hydrogen phosphate; (ii) the carboxymethylated cellulose matrix was stored in a saturated calcium hydroxide solution; (iii) the cellulose matrix was mixed with a calcium silicate solution in order to introduce silanol groups and to combine them with calcium ions. All the methods resulted in a mineralization of the cellulose surfaces after immersion in a simulated body fluid solution. Over a period of 14 days, the matrix was completely covered with hydroxyapatite crystals. Hydroxyapatite formation depended on functional groups on the matrix surface as well as on the precalcification method. The largest hydroxyapatite crystals were obtained on the carboxymethylated cellulose matrix treated with calcium hydroxide solution. The porous cellulose matrix was not cytotoxic, allowing the adhesion and proliferation of human osteoblastic cells. Comparatively, improved cell adhesion and growth rate were achieved on the mineralized cellulose matrices.
      pubtype: Academic Journal
      doctype:
        research
        Journal Article
      ougenre: Article
    language: English
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