Quantification of cerebellar hemispheric purkinje cell linear density: 32 ET cases versus 16 controls.

Although essential tremor (ET) is among the most prevalent neurological diseases, its precise pathogenesis is not understood. Purkinje cell loss has been observed in some studies and is the focus of interest and debate. Expressing these data as Purkinje cells/layer length allows one to adjust for th...

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Publicado en:Movement Disorders Vol. 28; no. 13; pp. 1854 - 1860
Autores principales: Louis, Elan D, Babij, Rachel, Lee, Michelle, Cortés, Etty, Vonsattel, Jean-Paul G
Formato: research Journal Article
Publicado: Wiley-Blackwell Nov2013
Acceso en línea:Ver este registro en EBSCOhost
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      dt: Nov2013
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      pub: Wiley-Blackwell
      place: Malden, Massachusetts
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        atl: Quantification of cerebellar hemispheric purkinje cell linear density: 32 ET cases versus 16 controls.
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        au:
          Louis, Elan D
          Babij, Rachel
          Lee, Michelle
          Cortés, Etty
          Vonsattel, Jean-Paul G
        affil: GH Sergievsky Center, College of Physicians and Surgeons, Columbia University, New York, NY, USA; Taub Institute for Research on Alzheimer's Disease and the Aging Brain, College of Physicians and Surgeons, Columbia University, New York, New York, USA; Department of Neurology, College of Physicians and Surgeons, Columbia University, New York, New York, USA; Department of Epidemiology, Mailman School of Public Health, Columbia University, New York, New York, USA.
      sug:
        subj:
          Cerebellum Pathology
          Essential Tremor Pathology
          Dominance, Cerebral
          Neurons Pathology
          Aged
          Aged, 80 and Over
          Calcium Binding Proteins Metabolism
          Cell Count
          Female
          Human
          Male
          Neurons Metabolism
          Nonparametric Statistics
          Aged: 65+ years
          Aged, 80 & over
          Female
          Male
      ab: Although essential tremor (ET) is among the most prevalent neurological diseases, its precise pathogenesis is not understood. Purkinje cell loss has been observed in some studies and is the focus of interest and debate. Expressing these data as Purkinje cells/layer length allows one to adjust for the inherent curved nature of the cerebellar folia. Capitalizing on the Essential Tremor Centralized Brain Repository, we quantified Purkinje cell linear density in cases versus controls. Free-floating 100-μm parasagittal cerebellar hemispheric sections were subjected to rabbit polyclonal anti-Calbindin D28k antibody, and 10 random fields/brain were selected for quantification of Purkinje cells/mm(-1) Purkinje cell layer. Purkinje cell linear density was lower in 32 ET cases than in16 controls (1.14 ± 0.32 vs. 1.35 ± 0.31/mm(-1) , P = 0.03). Purkinje cell linear density was inversely associated with torpedo count (r = -0.38, P = 0.028). The current sample of ET cases demonstrates a reduction in Purkinje cell number relative to that of controls. Greater Purkinje cell axonal remodeling (torpedoes) was found in individuals who had the most Purkinje cell drop out. The role of Purkinje cell loss in the pathogenesis of this disorder merits additional study.
      pubtype: Academic Journal
      doctype:
        research
        Journal Article
      ougenre: Article
    language: English
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