Immunization with a neural-derived Peptide protects the spinal cord from apoptosis after traumatic injury.
Apoptosis is one of the most destructive mechanisms that develop after spinal cord (SC) injury. Immunization with neural-derived peptides (INDPs) such as A91 has shown to reduce the deleterious proinflammatory response and the amount of harmful compounds produced after SC injury. With the notion tha...
| Publicado en: | BioMed Research International Vol. 2013; pp. 827517 - 827518 |
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| Autores principales: | , , , , , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Wiley-Blackwell
2013
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104115324&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104115324 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 2013 vid: 2013 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 104115324 104115324 2012373729 NLM24236295 PMC3819886 104115324 ppf: 827517 ppct: 1 formats: fmt: @attributes: type: P tig: atl: Immunization with a neural-derived Peptide protects the spinal cord from apoptosis after traumatic injury. aug: au: Rodríguez-Barrera, Roxana Fernández-Presas, Ana M García, Elisa Flores-Romero, Adrian Martiñón, Susana González-Puertos, Viridiana Yazmín Mestre, Humberto Flores-Dominguez, Carmina Rodriguez-Mata, Verónica Königsberg, Mina Solano, Sandra Ibarra, Antonio affil: Facultad de Ciencias de la Salud, Universidad Anáhuac México Norte, Edo. de México, CP 52786, Mexico ; Departamento de Ciencias de la Salud, División de Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana Iztapalapa, DF, CP 09340, Mexico ; Centro de Investigación del Proyecto CAMINA A.C., DF, CP 14050, Mexico ; Posgrado en Biología Experimental, Universidad Autónoma Metropolitana Iztapalapa, DF, CP 09340, Mexico. sug: subj: Apoptosis Drug Effects Immunization Nerve Tissue Proteins Pharmacodynamics Peptides Pharmacodynamics Spinal Cord Injuries Immunology Animal Studies Apoptosis Immunology Female Nerve Tissue Proteins Immunology Peptides Immunology Rats Spinal Cord Injuries Pathology Tumor Necrosis Factor Immunology Female ab: Apoptosis is one of the most destructive mechanisms that develop after spinal cord (SC) injury. Immunization with neural-derived peptides (INDPs) such as A91 has shown to reduce the deleterious proinflammatory response and the amount of harmful compounds produced after SC injury. With the notion that the aforementioned elements are apoptotic inducers, we hypothesized that INDPs would reduce apoptosis after SC injury. In order to test this assumption, adult rats were subjected to SC contusion and immunized either with A91 or phosphate buffered saline (PBS; control group). Seven days after injury, animals were euthanized to evaluate the number of apoptotic cells at the injury site. Apoptosis was evaluated using DAPI and TUNEL techniques; caspase-3 activity was also evaluated. To further elucidate the mechanisms through which A91 exerts this antiapoptotic effects we quantified tumor necrosis factor-alpha (TNF-α). To also demonstrate that the decrease in apoptotic cells correlated with a functional improvement, locomotor recovery was evaluated. Immunization with A91 significantly reduced the number of apoptotic cells and decreased caspase-3 activity and TNF-α concentration. Immunization with A91 also improved the functional recovery of injured rats. The present study shows the beneficial effect of INDPs on preventing apoptosis and provides more evidence on the neuroprotective mechanisms exerted by this strategy. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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