Molecular differences between chronic and aggressive periodontitis.
The 2 major forms of periodontitis, chronic (CP) and aggressive (AgP), do not display sufficiently distinct histopathological characteristics or microbiological/immunological features. We used molecular profiling to explore biological differences between CP and AgP and subsequently carried out super...
| Publicado en: | Journal of Dental Research Vol. 92; no. 12; pp. 1081 - 1089 |
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| Autores principales: | , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Sage Publications Inc.
Dec2013
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104116829&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104116829 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00220345 1H7 jtl: Journal of Dental Research issn: 00220345 maglogo: Y pubinfo: dt: Dec2013 vid: 92 iid: 12 pid: 344 pub: Sage Publications Inc. place: Thousand Oaks, California artinfo: ui: 104116829 NLM24122488 2012377310 10.1177/0022034513506011 NLM24122488 PMC3834653 104116829 ppf: 1081 ppct: 8 formats: tig: atl: Molecular differences between chronic and aggressive periodontitis. aug: au: Kebschull, M Guarnieri, P Demmer, R T Boulesteix, A L Pavlidis, P Papapanou, P N affil: Division of Periodontics, Section of Oral and Diagnostic Sciences, Columbia University College of Dental Medicine, New York, NY, USA. sug: subj: Aggressive Periodontitis Chronic Periodontitis Aggressive Periodontitis Immunology Aggressive Periodontitis Pathology Algorithms Apoptosis Pharmacokinetics Artificial Intelligence Chronic Periodontitis Metabolism Chronic Periodontitis Pathology Epithelium Pathology Gene Expression Profiling Methods Gingiva Pathology Human Microarray Analysis Periodontal Attachment Loss Periodontal Attachment Loss Pathology Periodontics Periodontal Pocket Periodontal Pocket Pathology ROC Curve Sensitivity and Specificity Signal Transduction RNA Gene Expression Profiling ab: The 2 major forms of periodontitis, chronic (CP) and aggressive (AgP), do not display sufficiently distinct histopathological characteristics or microbiological/immunological features. We used molecular profiling to explore biological differences between CP and AgP and subsequently carried out supervised classification using machine-learning algorithms including an internal validation. We used whole-genome gene expression profiles from 310 'healthy' or 'diseased' gingival tissue biopsies from 120 systemically healthy non-smokers, 65 with CP and 55 with AgP, each contributing with ≥ 2 'diseased' gingival papillae (n = 241; with bleeding-on-probing, probing depth ≥ 4 mm, and clinical attachment loss ≥ 3 mm), and, when available, a 'healthy' papilla (n = 69; no bleeding-on-probing, probing depth ≤ 4 mm, and clinical attachment loss ≤ 4 mm). Our analyses revealed limited differences between the gingival tissue transcriptional profiles of AgP and CP, with genes related to immune responses, apoptosis, and signal transduction overexpressed in AgP, and genes related to epithelial integrity and metabolism overexpressed in CP. Different classifying algorithms discriminated CP from AgP with an area under the curve ranging from 0.63 to 0.99. The small differences in gene expression and the highly variable classifier performance suggest limited dissimilarities between established AgP and CP lesions. Future analyses may facilitate the development of a novel, 'intrinsic' classification of periodontitis based on molecular profiling. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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