GRP78-targeted nanotherapy against castrate-resistant prostate cancer cells expressing membrane GRP78.
Glucose-regulated protein 78, GRP78, is a chaperone protein mainly located in the endoplasmic reticulum (ER) of normal cells. In stress conditions, GRP78 is overexpressed and in different cancer cell types, it is expressed at the cell surface, whereas it stays intracellular in non-cancerous cells. T...
| Publicado en: | Targeted Oncology Vol. 8; no. 4; pp. 225 - 231 |
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| Autores principales: | , , |
| Formato: | Journal Article |
| Publicado: |
Springer Nature
Dec2013
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104121210&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104121210 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 17762596 47DQ jtl: Targeted Oncology issn: 17762596 maglogo: N pubinfo: dt: Dec2013 vid: 8 iid: 4 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 104121210 NLM23090204 2012402176 10.1007/s11523-012-0234-9 NLM23090204 104121210 ppf: 225 ppct: 6 formats: fmt: @attributes: type: P tig: atl: GRP78-targeted nanotherapy against castrate-resistant prostate cancer cells expressing membrane GRP78. aug: au: Delie, Florence Petignat, Patrick Cohen, Marie affil: School of Pharmaceutical Sciences, University of Geneva, Quai Ernest Ansermet 30, 1211, Geneva 4, Switzerland. sug: subj: Proteins Metabolism Nanoparticles Paclitaxel Administration and Dosage Prostatic Neoplasms Drug Therapy Antibodies Administration and Dosage Antibodies Immunology Apoptosis Drug Effects Cell Line, Tumor Proteins Immunology Male Drug Therapy Methods Oligopeptides Immunology Prostatic Neoplasms Metabolism Prostatic Neoplasms Pathology Peptides Signal Transduction Male ab: Glucose-regulated protein 78, GRP78, is a chaperone protein mainly located in the endoplasmic reticulum (ER) of normal cells. In stress conditions, GRP78 is overexpressed and in different cancer cell types, it is expressed at the cell surface, whereas it stays intracellular in non-cancerous cells. Therefore, it appears as a strategic target to recognize malignant cells. Prostate cancer is one of the most diagnosed cancers in men. The development of castrate resistant tumors and the resistance to chemotherapy frequently occur. The carboxy-terminal ER retention domain is defined by the KDEL amino acid sequence. We developed anti-KDEL functionalized polymeric nanoparticles (NPs) loaded with paclitaxel (Tx) to specifically target prostate cancer cells expressing GRP78. The sensitivity to Tx in different formulations was compared in three prostate cell lines: PNT1B, a normal cell line, PC3, a cancer cell line faintly expressing GRP78 at its surface, and DU145, a cancer cell line expressing GRP78 at its cell surface. Our results show that the targeted formulation significantly increases Tx sensitivity of cell line expressing GRP78 at its surface compared to other treatments suggesting the added value of GRP78 targeted therapy for castrate resistant tumor which expresses GRP78 at its cell surface. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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