Use of magnetic folate-dextran-retinoic Acid micelles for dual targeting of Doxorubicin in breast cancer.
Amphiphilic copolymer of folate-conjugated dextran/retinoic acid (FA/DEX-RA) was self-assembled into micelles by direct dissolution method. Magnetic iron oxide nanoparticles (MNPs) coated with oleic acid (OA) were prepared by hydrothermal method and encapsulated within the micelles. Doxorubicin HCl...
| Published in: | BioMed Research International Vol. 2013; pp. 680712 - 680713 |
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| Main Authors: | , , , |
| Format: | equations & formulas pictorial research tables/charts Journal Article |
| Published: |
Wiley-Blackwell
2013
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104135930&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104135930 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 2013 vid: 2013 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 104135930 104135930 2012423578 NLM24381941 PMC3870081 104135930 ppf: 680712 ppct: 1 formats: fmt: @attributes: type: P tig: atl: Use of magnetic folate-dextran-retinoic Acid micelles for dual targeting of Doxorubicin in breast cancer. aug: au: Varshosaz, J Sadeghi-Aliabadi, H Ghasemi, S Behdadfar, B affil: Department of Pharmaceutics, School of Pharmacy and Novel Drug Delivery Systems Research Centre, Isfahan University of Medical Sciences, Isfahan 81745-359, Iran. sug: subj: Breast Neoplasms Drug Therapy Doxorubicin Administration and Dosage Drug Delivery Systems Ferric Compounds Administration and Dosage Breast Neoplasms Pathology Dextrans Administration and Dosage Dextrans Doxorubicin Female Ferric Compounds Folic Acid Administration and Dosage Folic Acid Human Cell Line, Tumor Colloids Tretinoin Administration and Dosage Tretinoin Female ab: Amphiphilic copolymer of folate-conjugated dextran/retinoic acid (FA/DEX-RA) was self-assembled into micelles by direct dissolution method. Magnetic iron oxide nanoparticles (MNPs) coated with oleic acid (OA) were prepared by hydrothermal method and encapsulated within the micelles. Doxorubicin HCl was loaded in the magnetic micelles. The characteristics of the magnetic micelles were determined by Fourier transform infrared (FT-IR) spectroscopy, thermogravimetric analysis (TGA), transmission electron microscopy (TEM), and vibrating sample magnetometer (VSM). The crystalline state of OA-coated MNPs and their heat capacity were analyzed by X-ray diffraction (XRD) and differential scanning calorimetry (DSC) methods, respectively. The iron content of magnetic micelles was determined using inductively coupled plasma optical emission spectrometry (ICP-OES). Bovine serum albumin (BSA) was used to test the protein binding of magnetic micelles. The cytotoxicity of doxorubicin loaded magnetic micelles was studied on MCF-7 and MDA-MB-468 cells using MTT assay and their quantitative cellular uptake by fluorimetry method. TEM results showed the MNPs in the hydrophobic core of the micelles. TGA results confirmed the presence of OA and FA/DEX-RA copolymer on the surface of MNPs and micelles, respectively. The magnetic micelles showed no significant protein bonding and reduced the IC50 of the drug to about 10 times lower than the free drug. pubtype: Academic Journal doctype: equations & formulas pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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