Deficient cytokine expression and neutrophil oxidative burst contribute to impaired cutaneous wound healing in diabetic, biofilm-containing chronic wounds.
Diabetic patients exhibit dysregulated inflammatory and immune responses that predispose them to chronic wound infections and the threat of limb loss. The molecular underpinnings responsible for this have not been well elucidated, particularly in the setting of wound biofilms. This study evaluates h...
| Publicado en: | Wound Repair & Regeneration Vol. 21; no. 6; pp. 833 - 842 |
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| Autores principales: | , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
Nov/Dec2013
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104150809&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104150809 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 10671927 DPV jtl: Wound Repair & Regeneration issn: 10671927 maglogo: Y pubinfo: dt: Nov/Dec2013 vid: 21 iid: 6 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 104150809 91719760 10.1111/wrr.12109 NLM24118295 104150809 ppf: 833 ppct: 9 formats: fmt: @attributes: type: P tig: atl: Deficient cytokine expression and neutrophil oxidative burst contribute to impaired cutaneous wound healing in diabetic, biofilm-containing chronic wounds. aug: au: Nguyen, Khang T. Seth, Akhil K. Hong, Seok J. Geringer, Matthew R. Xie, Ping Leung, Kai P. Mustoe, Thomas A. Galiano, Robert D. affil: Division of Plastic Surgery, Feinberg School of Medicine, Northwestern University sug: subj: Wound Healing Diabetes Mellitus Complications Biofilms Wounds and Injuries Physiopathology Chronic Disease Cytokines Neutrophils Animal Studies Mice Funding Source Models, Biological Microscopy, Electron, Scanning Biopsy T-Tests Analysis of Variance Data Analysis Software Descriptive Statistics ab: Diabetic patients exhibit dysregulated inflammatory and immune responses that predispose them to chronic wound infections and the threat of limb loss. The molecular underpinnings responsible for this have not been well elucidated, particularly in the setting of wound biofilms. This study evaluates host responses in biofilm-impaired wounds using the Tally Ho mouse, a clinically relevant polygenic model of type 2 diabetes. No differences in cytokine or Toll-like receptor ( TLR) expression were noted in unwounded skin or noninoculated wounds of diabetic and wild-type mice. However, diabetic biofilm-containing wounds had significantly less TLR 2, TLR 4, interleukin-1β, and tumor necrosis factor-α expression than wild-type wounds with biofilm (all p < 0.001). Both groups had similar bacterial burden and neutrophil infiltration after development of biofilms at 3 days postwounding, but diabetic wounds had significantly less neutrophil oxidative burst activity. This translated into a log-fold greater bacterial burden and significant delay of wound epithelization for biofilm-impaired diabetic wounds at 10 days postwounding. These results suggest that impaired recognition of bacterial infection via the TLR pathway leading to inadequate cytokine stimulation of antimicrobial host responses may represent a potential mechanism underlying diabetic susceptibility to wound infection and ulceration. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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