Hemagglutination and biofilm formation as virulence markers of uropathogenic Escherichia coli in acute urinary tract infections and urolithiasis.
Introduction: Urinary tract infections (UTI) are a major public health concern in developing countries. Most UTIs are caused by E. coli, accounting for up to 90% of community-acquired UTIs (CAUTI). Recurrent UTI is considered as a major risk factor for urolithiasis. Virulence factors like adhesins a...
| Publicado en: | Indian Journal of Urology Vol. 29; no. 4; pp. 277 - 282 |
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| Autores principales: | , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wolters Kluwer India Pvt Ltd
Oct-Dec2013
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104157125&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104157125 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 09701591 1KUG jtl: Indian Journal of Urology issn: 09701591 maglogo: N pubinfo: dt: Oct-Dec2013 vid: 29 iid: 4 pid: 16919 pub: Wolters Kluwer India Pvt Ltd artinfo: ui: 104157125 91942048 10.4103/0970-1591.120093 NLM24235787 PMC3822341 104157125 ppf: 277 ppct: 5 formats: fmt: @attributes: type: P tig: atl: Hemagglutination and biofilm formation as virulence markers of uropathogenic Escherichia coli in acute urinary tract infections and urolithiasis. aug: au: Maheswari, Uma B. Palvai, Sunitha Anuradha, Pattepu Rajalingam Kammili, Nagamani affil: Department of Microbiology, Gandhi Medical College and Hospital, Secunderabad, Andhra Pradesh, India sug: subj: Acute Disease Urinary Tract Infections Complications Urolithiasis Risk Factors Escherichia Coli Hemagglutination Tests Evaluation Biofilms Evaluation Virulence Evaluation Biological Markers Random Sample Human Chi Square Test Descriptive Statistics ab: Introduction: Urinary tract infections (UTI) are a major public health concern in developing countries. Most UTIs are caused by E. coli, accounting for up to 90% of community-acquired UTIs (CAUTI). Recurrent UTI is considered as a major risk factor for urolithiasis. Virulence factors like adhesins and biofilm have been extensively studied by authors on UPEC isolated from recurrent UTI.The studies on isolates from infection stones in kidney are scanty . In a prospective study, we aimed to determine the expression of Haemagglutinins, (Type 1 and P fimbriae) , Biofilm production and resistance pattern to common antibiotics of Uropathogenic E.coli (UPEC) isolates from Community acquired Acute Urinary Tract Infection(CAUTI) and Urolithiasis. Materials and Methods: A total of 43 UPEC isolates , 23 mid-stream urine (MSU) samples from patients with CAUTI attending Out Patient Departments and 20 from renal calculi of urolithiasis patients at the time of Percutaneous nephrolithostomy (PCNL ) were included in the study and the expression of Haemagglutinins,(Type 1 and P fimbriae) , Biofilm production and resistance pattern to common antibiotics was assessed. Results: A total of 43 UPEC isolates 23 from CAUTI and 20 from renal calculi were tested for production of biofilm and hemagglutinins. In CAUTI, biofilm producers were 56.52% and hemagglutinins were detected in all isolates 100%. In urolithiasis, biofilm producers were 100% but hemagglutinins were detected only in 70% of isolates. All isolates were resistant to multiple antibiotics used. CAUTI isolates were susceptible to 3rd generation cephalosporins, whereas urolithiasis isolates were resistant to 3rd generation cephalosporins and 25% were Extended Spectrum Beta Lactamases ESBL producers. Conclusions: HA mediated by type 1 fimbriae plays an important role in CAUTI ( P < 0.001 highly significant), whereas, in chronic conditions like urolithiasis, biofilm plays an important role in persistence of infection and the role of hemagglutinins is less. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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