Toll-like receptor stimulation increases phagocytosis of Cryptococcus neoformans by microglial cells.
Background: Toll-Like receptors (TLRs) belong to the family of pattern-recognition receptors with a crucial function of recognising pathogen-associated molecular patterns (PAMPs). Cryptococcal meningitis is a potentially fatal disease with a high mortality and risk of neurological sequelae.Methods:...
| Publicado en: | Journal of Neuroinflammation Vol. 10; no. 1; pp. 71 - 72 |
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| Autores principales: | , , , , |
| Formato: | research Journal Article |
| Publicado: |
BioMed Central
2013
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104165143&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104165143 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 17422094 1CUD jtl: Journal of Neuroinflammation issn: 17422094 maglogo: N pubinfo: dt: 2013 vid: 10 iid: 1 pid: 24147 pub: BioMed Central artinfo: ui: 104165143 104165143 NLM23738865 2012280861 10.1186/1742-2094-10-71 NLM23738865 PMC3693974 104165143 ppf: 71 ppct: 1 formats: tig: atl: Toll-like receptor stimulation increases phagocytosis of Cryptococcus neoformans by microglial cells. aug: au: Redlich, Sandra Ribes, Sandra Schütze, Sandra Eiffert, Helmut Nau, Roland affil: Institute of Neuropathology, University Medical Center, Göttingen, Germany. s.redlich77@web.de. sug: subj: Cryptococcus Immunology Cells Immunology Phagocytosis Drug Effects Receptors, Cell Surface Animals Animal Population Groups Cell Physiology Drug Effects Chemokines Metabolism Cytokines Peptides Pharmacodynamics Lipopolysaccharides Pharmacodynamics Mice Cells Drug Effects Carrier Proteins Metabolism Nucleotides Pharmacodynamics Signal Transduction Drug Effects Chemical and Pharmacologic Phenomena ab: Background: Toll-Like receptors (TLRs) belong to the family of pattern-recognition receptors with a crucial function of recognising pathogen-associated molecular patterns (PAMPs). Cryptococcal meningitis is a potentially fatal disease with a high mortality and risk of neurological sequelae.Methods: We studied the ability of microglial cells to increase the phagocytosis of cryptococci after stimulation with agonists of TLR1/2, TLR3, TLR4 and TLR9.Results: Stimulation of murine microglial cells with these TLR agonists for 24 h increased the phagocytosis of encapsulated Cryptococcus neoformans. Stimulation increased the release of TNF-α, CXCL1 (KC), IL-6, IL-10 and MIP-2, which indicated the activation of microglial cells. Unstimulated and TLR agonist-stimulated MyD88-deficient cells showed a reduced ability to phagocytose cryptococci compared to their wild-type counterpart. Intracellular killing of cryptococci was also increased in TLR-stimulated cells compared to unstimulated microglial cells.Conclusion: Our observation suggests that stimulation of microglial cells by TLR agonists can increase the resistance of the brain against CNS infections caused by Cryptococcus neoformans. This may be of interest when an immunocompromised patient is unable to eliminate Cryptococcus neoformans despite antifungal therapy. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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