Wound healing potential of topical bacteriophage therapy on diabetic cutaneous wounds.

Chronic wounds that fail to heal are a common complication of diabetes mellitus and the most common precipitating reason for nontraumatic lower limb amputation. Unfortunately, the bacterial species that cause these infections are becoming more resistant to antibiotics, making them increasingly diffi...

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Published in:Wound Repair & Regeneration Vol. 21; no. 4; pp. 595 - 604
Main Authors: Mendes, João J., Leandro, Clara, Corte-Real, Sofia, Barbosa, Raquel, Cavaco-Silva, Patrícia, Melo-Cristino, José, Górski, Andrzej, Garcia, Miguel
Format: pictorial research tables/charts Journal Article
Published: Wiley-Blackwell Jul/Aug2013
Online Access:View this record in EBSCOhost
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      dt: Jul/Aug2013
      vid: 21
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      pub: Wiley-Blackwell
      place: Malden, Massachusetts
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        atl: Wound healing potential of topical bacteriophage therapy on diabetic cutaneous wounds.
      aug:
        au:
          Mendes, João J.
          Leandro, Clara
          Corte-Real, Sofia
          Barbosa, Raquel
          Cavaco-Silva, Patrícia
          Melo-Cristino, José
          Górski, Andrzej
          Garcia, Miguel
        affil: TechnoPhage, S.A.; Internal Medicine Department, Santa Marta's Hospital / Central Lisbon Hospital Center, Lisbon
      sug:
        subj:
          Wound Healing
          Diabetic Foot Therapy
          Arboviruses
          Wounds, Chronic
          Animal Studies
          Swine
          Models, Biological
          Funding Source
          Rats
          Descriptive Statistics
          T-Tests
          Data Analysis Software
      ab: Chronic wounds that fail to heal are a common complication of diabetes mellitus and the most common precipitating reason for nontraumatic lower limb amputation. Unfortunately, the bacterial species that cause these infections are becoming more resistant to antibiotics, making them increasingly difficult to treat. We assessed the feasibility of combating chronic bacterial infections with a topically delivered bacteriophage cocktail in two animal models of diabetes mellitus. Microbiological, planimetric, and histological parameters were compared in debrided infected wounds with or without topical bacteriophage treatment. We determined that bacteriophage treatment effectively decreased bacterial colony counts and improved wound healing, as indicated by smaller epithelial and dermal gaps, in Staphylococcus aureus and Pseudomonas aeruginosa infections but was not as effective against Acinetobacter baumannii. Although the improvements were more significant in the rodent model than in the porcine model, our results suggest that topically administered bacteriophage treatment may be effective in resolving chronic infections, especially when applied in conjunction with wound debridement. These findings have important implications for the feasibility of using topical antimicrobial therapies to safely treat chronic infections in diabetes mellitus patients.
      pubtype: Academic Journal
      doctype:
        pictorial
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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