Methamphetamine alters blood brain barrier protein expression in mice, facilitating central nervous system infection by neurotropic Cryptococcus neoformans.

Methamphetamine (METH) is a drug of abuse that is a potent and highly addictive central nervous system (CNS) stimulant. The blood brain barrier (BBB) is a unique interface that in part functions to prevent microbial invasion of the CNS. The effects of METH on brain vasculature have not been studied...

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Published in:Journal of Infectious Diseases Vol. 208; no. 4; pp. 699 - 705
Main Authors: Eugenin, Eliseo A, Greco, Jade M, Frases, Susana, Nosanchuk, Joshua D, Martinez, Luis R
Format: research Journal Article
Published: Oxford University Press / USA Aug2013
Online Access:View this record in EBSCOhost
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        00221899
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      jtl: Journal of Infectious Diseases
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      dt: Aug2013
      vid: 208
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      pub: Oxford University Press / USA
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        2012199945
        10.1093/infdis/jit117
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      tig:
        atl: Methamphetamine alters blood brain barrier protein expression in mice, facilitating central nervous system infection by neurotropic Cryptococcus neoformans.
      aug:
        au:
          Eugenin, Eliseo A
          Greco, Jade M
          Frases, Susana
          Nosanchuk, Joshua D
          Martinez, Luis R
        affil: Public Health Research Institute.
      sug:
        subj:
          Blood-Brain Barrier
          Blood-Brain Barrier Drug Effects
          Cryptococcus Immunology
          Immunosuppressive Agents Adverse Effects
          Meningitis, Cryptococcal Immunology
          Methamphetamine Adverse Effects
          Proteins Drug Effects
          Animal Studies
          Brain Microbiology
          Cell Adhesion Molecules
          Central Nervous System Stimulants Adverse Effects
          Female
          Meningitis, Cryptococcal Mortality
          Mice
          Survival Analysis
          Female
      ab: Methamphetamine (METH) is a drug of abuse that is a potent and highly addictive central nervous system (CNS) stimulant. The blood brain barrier (BBB) is a unique interface that in part functions to prevent microbial invasion of the CNS. The effects of METH on brain vasculature have not been studied extensively. We hypothesized that METH alters the BBB integrity, increasing susceptibility to CNS infection. Using a murine model of METH administration, we demonstrated that METH alters BBB integrity and modifies the expression of tight junction and adhesion molecules. Additionally, we showed that BBB disruption accelerates transmigration of the neurotropic fungus Cryptococcus neoformans into the brain parenchyma after systemic infection. Furthermore, METH-treated mice displayed increased mortality as compared to untreated animals. Our findings provide novel evidence of the impact of METH abuse on the integrity of the cells that comprise the BBB and protect the brain from infection.
      pubtype: Academic Journal
      doctype:
        research
        Journal Article
      ougenre: Article
    language: English
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