Molecular characterization of the circulating strains of Vibrio cholerae during 2010 cholera outbreak in Nigeria.
This study aimed at characterizing the phenotypic and toxigenic status of circulating strains of cholera during outbreaks in Nigeria, employing molecular typing techniques. Two hundred and one samples of rectal swabs, stool, vomitus, water (from the well, borehole, sachet, stream, and tap) and disin...
| Publicado en: | Journal of Health, Population & Nutrition Vol. 31; no. 2; pp. 178 - 185 |
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| Autores principales: | , , , , , , , , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
BioMed Central
Jun2013
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104208713&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104208713 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 16060997 HII jtl: Journal of Health, Population & Nutrition issn: 16060997 maglogo: N pubinfo: dt: Jun2013 vid: 31 iid: 2 pid: 24147 pub: BioMed Central artinfo: ui: 104208713 104208713 NLM23930335 2012221435 NLM23930335 PMC3702338 104208713 ppf: 178 ppct: 7 formats: fmt: @attributes: type: P tig: atl: Molecular characterization of the circulating strains of Vibrio cholerae during 2010 cholera outbreak in Nigeria. aug: au: Oyedeji, Kolawole S Niemogha, Mary-Theresa Nwaokorie, Francisca O Bamidele, Tajudeen A Ochoga, Michael Akinsinde, Kehinde A Brai, Bartholomew I Oladele, David Omonigbehin, Emmanuel A Bamidele, Moses Fesobi, Toun W Musa, Adesola Z Adeneye, Adeniyi K Smith, Stella I Ujah, Innocent A affil: Molecular Biology and Biotechnology Division, Nigerian Institute of Medical Research, Yaba, Lagos. sug: subj: Cholera Epidemiology Disease Outbreaks Genetic Techniques Methods Gram-Negative Bacteria Cholera Blood DNA Human Nigeria Polymerase Chain Reaction Methods ab: This study aimed at characterizing the phenotypic and toxigenic status of circulating strains of cholera during outbreaks in Nigeria, employing molecular typing techniques. Two hundred and one samples of rectal swabs, stool, vomitus, water (from the well, borehole, sachet, stream, and tap) and disinfectants (sodium hypochlorite) were collected from three states in the country. The samples were inoculated on thiosulphate-citrate bile salt-sucrose (TCBS), Cary-Blair transport medium and smeared on glass slides for direct examination. The Vibrio cholerae isolates were serotyped, biotyped, and characterized using PCR of the cytotoxin gene A (ctxA), wbeO1, and wbfO139 gene primer. Of the 201 samples screened, 96 were positive for V cholerae O1 (48%), with 69 (72%) positive for ctxA gene. The results from this study showed that the circulating strains of cholera in Nigeria were of Ogawa serotype, also observed in other outbreaks in Nigeria (1991, 1992, and 1996). However, the strains were of the Classical biotype and were mainly (72%) ctxA gene-positive. This current investigation has confirmed the production of cholera toxin by the circulating strains, and this could be harnessed for possible cholera vaccine production in Nigeria. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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