Adipose tissue-derived stem cells ( ADSCs) transplantation promotes regeneration of expanded skin using a tissue expansion model.

Transplantation of adipose tissue-derived stem cells ( ADSCs) is a promising method that has been used in regenerative medicine because it has shown the capacity to accelerate wound healing. However, roles of ADSCs transplantation in expanded-skin regeneration have remained unknown. To clarify the r...

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Publicado en:Wound Repair & Regeneration Vol. 21; no. 5; pp. 746 - 755
Autores principales: Sheng, Lingling, Yang, Mei, Liang, Yimin, Li, Qingfeng
Formato: pictorial research tables/charts Journal Article
Publicado: Wiley-Blackwell Sep/Oct2013
Acceso en línea:Ver este registro en EBSCOhost
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      dt: Sep/Oct2013
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      pub: Wiley-Blackwell
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        atl: Adipose tissue-derived stem cells ( ADSCs) transplantation promotes regeneration of expanded skin using a tissue expansion model.
      aug:
        au:
          Sheng, Lingling
          Yang, Mei
          Liang, Yimin
          Li, Qingfeng
        affil: Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine
      sug:
        subj:
          Adipose Tissue Physiology
          Stem Cells Transplantation
          Regeneration
          Animal Studies
          Funding Source
          Rats
          Models, Biological
          Flow Cytometry
          Growth Substances Analysis
      ab: Transplantation of adipose tissue-derived stem cells ( ADSCs) is a promising method that has been used in regenerative medicine because it has shown the capacity to accelerate wound healing. However, roles of ADSCs transplantation in expanded-skin regeneration have remained unknown. To clarify the roles, a tissue expansion model was used in this study. The study comprised three groups of 13 rats in each group: the ADSCs group, the fibroblast ( FB) group, and the control group. The skin regeneration in the ADSCs group was enhanced, as evidenced by increased cell proliferation and a higher hydroxyproline content and degree of neovascularization, all with p < 0.05, when compared with both the FB group and the control group. Consistent with enhanced cell proliferation and neovascularization, the regenerated skin in the ADSCs group was much thicker, which further reduced the retraction ratio of the expanded skin. Four weeks after operation, 5'-bromo-2'-deoxyuridine-labeled ADSCs appeared in subcutaneous tissue, vascular vessels, and hair follicles. The up-regulation of protein expression, such as epidermal growth factor and vascular endothelial growth factor, primarily emerged in the ADSC group, with the up-regulated basic fibroblast growth factor appearing in the FB group. Collectively, these results suggest that the transplantation of ADSCs could enhance the regeneration of expanded skin by participating in skin structures and up-regulating the secretion of epidermal growth factor and vascular endothelial growth factor.
      pubtype: Academic Journal
      doctype:
        pictorial
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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