Evaluation of a new fluorescence quantitative PCR test for diagnosing Helicobacter pylori infection in children.
Background: Numerous diagnostic tests are available to detect Helicobactor pylori (H. pylori). There has been no single test available to detect H. pylori infection reliably. We evaluated the accuracy of a new fluorescence quantitative PCR (fqPCR) for H. pylori detection in children.Methods: Gastric...
| Publicado en: | BMC Gastroenterology Vol. 13; no. 1; pp. 7 - 8 |
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| Autores principales: | , , , , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
BioMed Central
2013
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104225321&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104225321 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 1471230X 1CHO jtl: BMC Gastroenterology issn: 1471230X maglogo: N pubinfo: dt: 2013 vid: 13 iid: 1 pid: 24147 pub: BioMed Central artinfo: ui: 104225321 104225321 NLM23311469 2012261178 10.1186/1471-230X-13-7 NLM23311469 PMC3598558 104225321 ppf: 7 ppct: 1 formats: tig: atl: Evaluation of a new fluorescence quantitative PCR test for diagnosing Helicobacter pylori infection in children. aug: au: Ou, Zhiying Xiong, Liya Li, Ding-You Geng, Lanlan Li, Lixia Chen, Peiyu Yang, Min Zeng, Yongmei Zhou, Zhenwen Xia, Huimin Gong, Sitang affil: Department of Gastroenterology, Guangzhou Women and Children's Medical Center, Guangzhou Medical College, 9 Jinsui Road, Guangzhou, 510623, China. dyli@cmh.edu. sug: subj: Light Gastritis Diagnosis Gastritis Microbiology Helicobacter Infections Diagnosis Helicobacter Pylori Polymerase Chain Reaction Methods Adolescence Biopsy Breath Tests Child Child, Preschool Dental Plaque Metabolism Female Gastric Juice Metabolism Gastric Mucosa Microbiology Gastric Mucosa Pathology Gastritis Metabolism Helicobacter Infections Metabolism Human Male Prospective Studies Sensitivity and Specificity Stomach Microbiology Stomach Pathology Urease Metabolism Adolescent: 13-18 years Child: 6-12 years Child, Preschool: 2-5 years Female Male ab: Background: Numerous diagnostic tests are available to detect Helicobactor pylori (H. pylori). There has been no single test available to detect H. pylori infection reliably. We evaluated the accuracy of a new fluorescence quantitative PCR (fqPCR) for H. pylori detection in children.Methods: Gastric biopsy specimens from 138 children with gastritis were sent for routine histology exam, rapid urease test (RUT) and fqPCR. 13C-urea breath test (13C-UBT) was carried out prior to endoscopic procedure. Gastric fluids and dental plaques were also collected for fqPCR analysis.Results: 38 children (27.5%) were considered positive for H. pylori infection by gold standard (concordant positive results on 2 or more tests). The remaining 100 children (72.5%) were considered negative for H. pylori. Gastric mucosa fqPCR not only detected all 38 H. pylori positive patients but also detected 8 (8%) of the 100 gold standard-negative children or 11 (10.7%) of the 103 routine histology-negative samples. Therefore, gastric mucosa fqPCR identified 46 children (33.3%) with H. pylori infection, significantly higher than gold standard or routine histology (P<0.01). Both gastric fluid and dental plaque fqPCR only detected 32 (23.2%) and 30 (21.7%) children with H. pylori infection respectively and was significantly less sensitive than mucosa fqPCR (P<0.05) but was as sensitive as non-invasive UBT.Conclusions: Gastric mucosa fqPCR was more sensitive than routine histology, RUT, 13C-UBT alone or in combination to detect H. pylori infection in children with chronic gastritis. Either gastric fluid or dental plaque PCR is as reliable as 13C-UBT for H. pylori detection. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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