Low rate of recurrence of Helicobacter pylori infection in spite of high clarithromycin resistance in Pakistan.

Background: The aim was to investigate the reinfection rate of H. pylori during a follow-up period of 12 months in adults who had undergone eradication therapy.Methods: One hundred-twenty patients; 116 with gastritis, 3 with duodenal ulcer and 1 gastric ulcer, were studied. Their mean age was 41±13...

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Publicado en:BMC Gastroenterology Vol. 13; no. 1; pp. 33 - 34
Autores principales: Yakoob, Javed, Abid, Shahab, Jafri, Wasim, Abbas, Zaigham, Mumtaz, Khalid, Hamid, Saeed, Ahmed, Rashida
Formato: research Journal Article
Publicado: BioMed Central 2013
Acceso en línea:Ver este registro en EBSCOhost
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      dt: 2013
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        10.1186/1471-230X-13-33
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        atl: Low rate of recurrence of Helicobacter pylori infection in spite of high clarithromycin resistance in Pakistan.
      aug:
        au:
          Yakoob, Javed
          Abid, Shahab
          Jafri, Wasim
          Abbas, Zaigham
          Mumtaz, Khalid
          Hamid, Saeed
          Ahmed, Rashida
        affil: Department of Medicine, Aga Khan University, Stadium Road, Karachi, 74800, Pakistan. shahab.abid@aku.edu.
      sug:
        subj:
          Antibiotics Therapeutic Use
          Clarithromycin Therapeutic Use
          Drug Resistance, Microbial
          Helicobacter Infections Drug Therapy
          Helicobacter Infections Epidemiology
          Helicobacter Pylori
          Adolescence
          Adult
          Aged
          Amoxicillin Therapeutic Use
          Female
          Prospective Studies
          Gastritis Microbiology
          Human
          Male
          Middle Age
          Pakistan
          Peptic Ulcer Microbiology
          Prevalence
          Recurrence
          Treatment Outcomes
          Young Adult
          Adolescent: 13-18 years
          Adult: 19-44 years
          Aged: 65+ years
          Middle Aged: 45-64 years
          Female
          Male
      ab: Background: The aim was to investigate the reinfection rate of H. pylori during a follow-up period of 12 months in adults who had undergone eradication therapy.Methods: One hundred-twenty patients; 116 with gastritis, 3 with duodenal ulcer and 1 gastric ulcer, were studied. Their mean age was 41±13 years (range 18-77) and male: female ratio of 2:1. H. pylori were cultured and antibiotic sensitivity was determined by Epsilometer test (E-test) for clarithromycin (CLR) and amoxicillin (AMX). Primers of urease C gene of H. pylori and Sau-3 and Hha I restriction enzymes were used for polymerase chain reaction-restriction fragment length polymorphism analysis (PCR-RFLP). 14C urea breath test (14C-UBT) was performed 4 weeks after the eradication therapy. The successfully treated patients were observed for 12 months with 14C-UBT to assess H. pylori status. If 14C-UBT was negative, it was repeated after every 12 weeks. If 14C-UBT was positive, endoscopy was repeated with biopsies.Result: The eradication therapy was successful in 102(85%) patients. Out of forty-seven H. pylori isolates cultured, clarithromycin sensitivity was present in 30(64%) and amoxicillin in 45(98%), respectively. Follow-up 14C-urea breath tests of all 102 patients who eradicated H. pylori remained negative up to 9 months. However, in 6 patients, the 14C-UBT confirmed recurrence at 12 months. The recurrence rate was 6%.Conclusion: A low rate of recurrence of H. pylori infection was found in patients with dyspeptic symptoms. H. pylori isolates demonstrated a high invitro clarithromycin resistance.
      pubtype: Academic Journal
      doctype:
        research
        Journal Article
      ougenre: Article
    language: English
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