Comparison of the Virulence Markers of Helicobacter Pylori and their Associated Diseases in Patients from Pakistan and Afghanistan.
Background/Aim: Helicobacter pylori is a Gram-negative bacteria, which is associated with development of gastroduodenal diseases. The prevalence of H. pylori and the virulence markers cytotoxin-associated gene A and E (cagA, cagE) and vacuolating-associated cytotoxin gene (vacA) alleles varies in di...
| Publicado en: | Saudi Journal of Gastroenterology Vol. 19; no. 5; pp. 211 - 219 |
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| Autores principales: | , , , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Wolters Kluwer India Pvt Ltd
Sep2013
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104229374&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104229374 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 13193767 322P jtl: Saudi Journal of Gastroenterology issn: 13193767 maglogo: N pubinfo: dt: Sep2013 vid: 19 iid: 5 pid: 16919 pub: Wolters Kluwer India Pvt Ltd artinfo: ui: 104229374 90400924 10.4103/1319-3767.118123 104229374 ppf: 211 ppct: 8 formats: tig: atl: Comparison of the Virulence Markers of Helicobacter Pylori and their Associated Diseases in Patients from Pakistan and Afghanistan. aug: au: Yakoob, Javed Abbas, Zaigham Jafri, Wasim Usman, Muhammad W. Jafri, Fatima Awan, Safia affil: Department of Medicine, Aga Khan University, Karachi, Pakistan sug: subj: Helicobacter Pylori Biological Markers Virulence Human Pakistan Afghanistan Polymerase Chain Reaction Utilization Stomach Neoplasms Diagnosis Male Female Adult Middle Age Descriptive Statistics Biopsy Utilization Stomach Pathology Data Analysis Software Genotype Funding Source Adult: 19-44 years Middle Aged: 45-64 years Male Female ab: Background/Aim: Helicobacter pylori is a Gram-negative bacteria, which is associated with development of gastroduodenal diseases. The prevalence of H. pylori and the virulence markers cytotoxin-associated gene A and E (cagA, cagE) and vacuolating-associated cytotoxin gene (vacA) alleles varies in different parts of the world. H. pylori virulence markers cagA, cagE, and vacA alleles in local and Afghan nationals with H. pylori-associated gastroduodenal diseases were studied. Patients and Methods: Two hundred and ten patients with upper gastrointestinal symptoms and positive for H. pylori by the urease test and histology were included. One hundred and nineteen were local nationals and 91 were Afghans. The cagA, cagE, and vacA allelic status was determined by polymerase chain reaction. Results: The nonulcer dyspepsia (NUD) was common in the Afghan patients (P = 0.025). In Afghan H. pylori strains, cagA was positive in 14 (82%) with gastric carcinoma (GC) compared with 29 (45%) with NUD (P = 0.006), whereas cagE was positive in 11 (65%) with GC and 4 (67%) with duodenal ulcer (DU) compared with 12 (18%) with NUD (P < 0.001 and 0.021, respectively). The vacA s1a/b1was positive in 10 (59%) of GC compared with 20 (31%) in NUD (P = 0.033). In Pakistani strains, cagE was positive in 12 (60%) with GC, 7 (58%) with GU, 12 (60%) with DU compared with 11 (16%) with NUD (P < 0.001, 0.004, and < 0.001, respectively). In Pakistani strains, cagA/s1a/m1 was 39 (33%) compared with Afghans in 17 (19%) (P = 0.022). Moderate to severe mucosal inflammation was present in 51 (43%) Pakistani patients compared with 26 (28%) (P = 0.033) in Afghans. It was also associated with grade 1 lymphoid aggregate development in Pakistani patients 67 (56%) compared with 36 (40%) (P = 0.016) in Afghans. Conclusion: Distribution of H. pylori virulence marker cagE with DU was similar in Afghan and Pakistan H. pylori strains. Chronic active inflammation was significantly associated with Pakistani H. pylori strains. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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